Nugc2

We used GC cell lines, which were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) as follows: MKN1, MKN45, MKN74, NUGC2, NUGC3, NUGC4, and SC-6-JCK. The GC cell lines AGS, KATOIII, and N87 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The human intestinal epithelial cell line FHs74Int (ATCC) served as a nontumorigenic control. Primary GC tissues and corresponding noncancerous mucosal tissues were collected from 200 patients who underwent gastrectomy at Nagoya University Hospital between 2001 and 2014. None of the patients underwent preoperative chemotherapy.
The methods were carried out in accordance with relevant guidelines. The study protocol was approved by the Medical Ethics Committee of the Nagoya University Hospital, protocol No. 2014–0043. Informed consent was obtained from all patients. Written informed consent for the use of clinical samples and data, as required by the Institutional Review Board, was obtained from all patients.

GC cell lines, the differentiated type (AGS, IM95, MKN1, MKN7, MKN74 and N87) and the undifferentiated type (GCIY, KATO-III, MKN45, NUGC2, NUGC3, NUGC4, OCUM1 and SC-6-JCK), were obtained from the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan) or the American Type Culture Collection (ATCC, Manassas, VA, USA). A control, non-tumorigenic epithelial cell line (FHs 74) was purchased from the ATCC. The cells were cultured at 37 °C in RPMI medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% foetal bovine serum in an atmosphere containing 5% CO₂. All cell lines were authenticated using the short tandem repeat PCR method by the Japanese Collection of Research Bio Resources Cell Bank before the study commenced.

The GC cell lines GCIY, IM95, MKN1, MKN7, MKN45, MKN74, NUGC2, NUGC3, NUGC4, OCUM-1, and SC-6-JCK were obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). AGS, KATOIII, and N87 GC cell lines and a nontumorigenic epithelial cell line (FHs74) were acquired from the American Type Culture Collection (Manassas, VA, USA). Three hundred pairs of surgically resected GC and adjacent noncancerous tissues were obtained from patients who underwent gastrectomy. A freely available integrated dataset (n = 1065 GC patients) was accessed at http://kmplot.com/analysis/ [13 (link)].