Real time pcr rt pcr primers

Fractalkine antibodies were purchased from Abcam (Cambridge, MA). Antibodies against IBA1 were purchased from Wako Chemicals USA (Richmond, VA). NeuN antibodies were ordered from Millipore (Billerica, MA). Antibodies against CD11b and glutamate aspartate transporter (GLAST) were purchased from Miltenyi Biotec (San Diego, CA). Phosphorylated and total extracellular signal-regulated kinase 1/2 (pERK1/2 and tERK1/2) antibodies were purchased from Cell Signaling (Danvers, MA). Mouse soluble fractalkine was purchased from R&D Systems (Minneapolis, MN). Mouse fracktalkine, CCL2, IL-6, and TNFα ELISAs were purchased from R&D Systems. All real-time PCR (RT-PCR) primers were purchased from SABiosciences (Frederick, MD). Total bilirubin was assayed using an ELISA kit from Cusabio (Wuha, China). All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted and were of the highest grade available.

Antibodies against IBA1 were purchased from Wako Chemicals USA (Richmond, VA, USA). Antibodies against CCL2, CCR2, and CCR4 were purchased from Genetex (Irvine, CA, USA). NeuN antibodies were ordered from Millipore (Billerica, MA, USA). All real-time PCR (RT-PCR) primers were purchased from SABiosciences (Frederick, MD, USA). INCB 3284 dimesylate (INCB) and C021 dihydrochloride (C021) were purchased from Tocris Bioscience (Minneapolis, MN, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted, and were of the highest grade available.