Rabbit anti human p53 antibody

Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). Dextran sodium sulfate (DSS) was purchased from MP Biomedicals, LLC (Santa Ana, CA, USA). The ELISA kits for TNF-α (cat. no. A0119), IL-6 (cat. no. A0107) were purchased from Nanjing Jiancheng Bioengineering Institute (China). All plastic materials were purchased from Falcon Labware (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). RPMI Medium-1640, fetal bovine serum (FBS), and phosphate buffer saline (PBS) were obtained from Gibco (Thermo Fisher Scientific, Inc. Waltham, MA, USA). Penicillin G/streptomycin, MTT, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Merck KGaA Darmstadt, Germany). Apoptosis Assay kit was purchased from Nanjing KeyGen Biological Co., Ltd. (Nanjing, China). Rabbit anti-human Caspase antibody (cat no. 9661; 1:1,000; Cell Signaling Technology, Inc.), rabbit anti-human BAX antibody (cat no. 2774; 1:1,000; Cell Signaling Technology, Inc.), rabbit anti-human p53 antibody (cat no. 48818; 1:1,000; Cell Signaling Technology, Inc.) and rabbit anti-human β-actin antibody (cat no. 622101; 1:2,000) were purchased from BioLegend Inc. (San Diego, CA, USA). Human colon cancer epithelial cells (HT-29) were purchased from the Chinese Academy of Sciences Shanghai Cell Bank (Shanghai, China).