Cyanine5.5 nhs ester

2(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate (HBTU) and the N-hydroxybenzotriazole (HOBt) were purchased from Vivitide (Gardner, MA). All protected amino acids, rink amide MBHA resin, and N-methylmorpholine (NMM) were supplied by Gyros Protein Technologies (Tucson, AZ). Polyethylene glycol (PEG₃) was obtained from Creative PEGWorks (Durham, NC). Trifluoroacetic acid (TFA), piperidine, thioanisole, anisole, 1,2-ethanedithiole, methyl-tert-butyl ether, N,N-diisopropylethylamine (DIPEA), dimethylformamide (DMF), acetonitrile (ACN), N-methyl-2-pyrrolidone (NMP), sodium carbonate, Sephadex G25, L-glutathione reduced (GSH), lipopolysaccharide (LPS), and cis-dichlorodiammine platinum (II) (cisplatin) were purchased from Sigma Aldrich (Saint-Louis, MO). Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) was supplied by Invitrogen (Carlsbad, CA). p-SNC-deferoxamine (DFO) was from Macrocyclics Inc. (Plato, TX). N2’-deacetyl-N2’-(3-mercapto-1-oxopropyl)-maytansine (DM1) and aldoxorubicin HCl (aldox) were from MedKoo Biosciences (Morrisville, NC). Doxorubicin (DOX) and gemcitabine (GEM) were obtained from LC Laboratories (Woburn, MA). Mitomycin (MIT) was supplied by Selleckchem (Houston, TX). Cyanine5.5 NHS ester was purchased from Lumiprobe Corporation (Hallandale Beach, FL) and luciferin was from Caliper LifeScience (Hopkinton, MA).

Rink-amide resin, protected amino acids, and solvents used for peptide synthesis were obtained from Protein Technologies Inc. (Tucson, AZ). Fluorescein isothiocyanate (FITC), trifluoroacetic acid, thioanisole, anisole, 1,2-ethanedithiole, methyl-tert-butyl ether, N,N-diisopropylethylamine, hydrazine, GSH, MA, and 3MA were purchased from Sigma-Aldrich (Saint-Louis, MO). Sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate (sulfo-SMCC) was obtained from Novachem (Calgary, Canada) and p-SNC-deferoxamine (DFO) was from Macrocyclics Inc. (Plato, TX). AlDoxorubicin was purchased from Medkoo Biosciences (Chapel Hill, NC). Doxorubicin was from LC Laboratories (Woburn, MA), and N2′- deacetyl-N2′-(3-mercapto-1-oxopropyl)-maytansine was supplied by Carbosynth Ltd (Compton, WB). Uranyl formate was purchased from Electron Microscopy Sciences (Hatfield, PA), and 4′6-diaminidino-2-pheylindole (DAPI) and LysoTracker Red were purchased from Life Technologies Inc. (Norwalk, CT). Cyanine5.5 NHS ester was obtained from Lumiprobe Corporation (Hallandale Beach, FL) and luciferin was supplied by Caliper LifeSciences (Hopkinton, MA).

Fmoc-protected amino acids, resins, and coupling reagents for solid-phase synthesis were purchased from EMD Millipore. Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) was purchased from Sigma-Aldrich. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-mPEG1000 (mPEG1000-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG2000-azide (N₃-PEG2000-DSPE), and cholesterol (Chol) were purchased from Avanti Polar Lipids. Cyanine 5.5-NHS ester was purchased from Lumiprobe. Cellulose acetate (CA) syringe filters were purchased from Macherey-Nagel Inc. Sephadex^® G-50 and LH-20 were purchased from GE Healthcare. LIVE/DEAD^® fixable green dead cell stain was purchased from ThermoFisher. AlexaFluor^® 680-Wheat Germ Agglutinin conjugate (WGA) and LysoTracker^® Blue DND-22 were purchased from Life Technologies. Cell culture reagents such as DMEM, FBS, trypsin, and PBS were purchased from Atlanta Biologicals. Ultrapure water (18 MΩ) was used for preparation of all buffers and in all experiments. All solvents were of analytical grade, purchased from commercial sources and used without further purification, except DMF which was dried over CaH₂ under N₂, filtered, and distilled under reduced pressure.