MB (Proveblue®) was a gift from Provepharm Life Solutions (Marseilles, France). diC16dT (CAS number: 1160002-70-9) and DOTAU (CAS number: 868226-06-6) were synthesized in house according to previous publication.20,21,24 (link) Chloroquine (CQ), quinine (QN), dihydroartemisinin (DHA) and doxycycline (DOX) were purchased from Sigma (Saint Louis, MO, USA). Amodiaquine (DQ) was provided by the WHO (Geneva, Switzerland) and mefloquine (MQ) was a gift from Roche (Paris, France). Lumefantrine (LMF) from Novartis Pharma (Basel, Switzerland), artesunate (AS), piperaquine (PPQ) and pyronaridine (PND) were purchased from Shin Poong Pharm Co. (Seoul, Korea). Absolute ethanol (EtOH) was purchased from VWR Chemicals (France). Water used in all experiments except in vitro experiment was produced in-house by ultrapure water system (ELGA Millipore system, minimum resistivity: 18.2 MΩ) and subsequently distilled to prevent any possible trace of resin from the ultrapure water system. MilliQ was purchased from Merck Millipore (MA, USA). RPMI (Roswell Park Memorial Institute) 1640 medium was purchased from Sigma Aldrich (France). Ascorbic acid and NaCl were purchased from COOPER (France). P. falciparum chloroquine-susceptible strains 3D7 (African) was obtained from MR4 (VA, USA) and multi-drug resistant W2 strain (Indochina) was obtained from MR4 (VA, USA).
Absolute ethanol etoh
Manufactured by Avantor
Sourced in France, United States, Spain
Absolute ethanol (EtOH) is a clear, colorless, and flammable liquid chemical compound. It is used as a solvent, disinfectant, and as a fuel additive. Absolute ethanol is a highly purified form of ethanol, containing a minimum of 99.5% ethanol.
Automatically generated - may contain errors
Lab products found in correlation
Milli q, by Merck Group (5 mentions)
Tetraethyl orthosilicate, by Merck Group (2 mentions)
Glacial acetic acid, by Avantor (2 mentions)
Sodium hydroxide (naoh), by ITW Reagents (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Roswell park memorial institute (rpmi), by Merck Group (1 mentions)
Doxycycline dox, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Alginic acid sodium salt from brown algae, by Merck Group (1 mentions)
Calcium chloride anhydrous, by Scharlab (1 mentions)
Ammonium hydroxide solution, by Merck Group (1 mentions)
Calcium nitrate tetrahydrate, by Merck Group (1 mentions)
Copper nitrate trihydrate, by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
Alginic acid sodium salt, by Merck Group (1 mentions)
Glutaraldehyde, by Scharlab (1 mentions)
Potassium persulfate k2s2o8, by Merck Group (1 mentions)
13 protocols using absolute ethanol etoh
1
Antimalarial Compounds Characterization
2
Synthesis and Characterization of Alginic Acid-Vancomycin Nanocarriers
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Alginic acid sodium salt from brown algae (guluronic/mannuronic acid ratio of 70/30) was supplied by Sigma Life Science (Irvine, UK). Vancomycin hydrochloride (C66H75Cl2N9O24·HCl, 94.3% purity) was supplied by Guinama (Valencia, Spain). Calcium chloride anhydrous (CaCl2, >99% purity) was supplied by Scharlab (Barcelona, Spain). Absolute ethanol (EtOH, >99.9% purity) and CO2 (99.8% purity) were purchased from VWR Chemicals (Fontenay-sous-Bois, France) and Nippon Gases (Madrid, Spain), respectively. Tetraethylorthosilicate (TEOS, 98% purity), ammonium aqueous solution 30–33% and 1H,1H,2H,2H-perfluorodecyltriethoxisilane (PFDTS, 97% purity) were from Sigma–Aldrich (Darmstadt, Germany). Water was purified using reverse osmosis (resistivity > 18 MΩ·cm, Milli-Q, Millipore®, Madrid, Spain).
Ultrapure nitrogen (N2 (g), >99% purity) supplied by Praxair (Madrid, Spain) was used for the adsorption-desorption textural analysis. Phosphate buffered saline (PBS) pH 7.4, and potassium dihydrogen phosphate (KH2PO4, 98.0–100.5% purity) were both supplied by ITW Reagents (Barcelona, Spain). Sodium hydroxide (NaOH, 99% purity) and acetonitrile (CH3CN, ≥99.9% purity) were both purchased from VWR Chemicals (Barcelona, Spain).
Ultrapure nitrogen (N2 (g), >99% purity) supplied by Praxair (Madrid, Spain) was used for the adsorption-desorption textural analysis. Phosphate buffered saline (PBS) pH 7.4, and potassium dihydrogen phosphate (KH2PO4, 98.0–100.5% purity) were both supplied by ITW Reagents (Barcelona, Spain). Sodium hydroxide (NaOH, 99% purity) and acetonitrile (CH3CN, ≥99.9% purity) were both purchased from VWR Chemicals (Barcelona, Spain).
3
Sol-Gel Synthesis of Silica-Based Materials
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Tetraethyl orthosilicate (TEOS, 99%), cetyltrimethylammonium bromide (CTAB, 98%), ammonium hydroxide solution (NH3, 28–30%), calcium nitrate tetrahydrate (Ca(NO3)2·4H2O, 99%), and copper nitrate trihydrate (Cu(NO3)2·3H2O, 99–104%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Absolute ethanol (EtOH, 99.5%) was obtained from VWR Chemicals. Deionized water was used in all experiments.
4
Alginate-Hydroxyapatite Composite Fabrication
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Alginic acid sodium salt from brown algae with medium viscosity (guluronic acid/mannuronic acid ratio: 70/30, Mw 403 kDa, 3170 cps) and calcium chloride (CaCl2; Mw 110.98 g/mol, 99.99% purity) were provided by Sigma Aldrich (Madrid, Spain). Hydroxyapatite (HA; Mw 502.31 g/mol, reagent grade purity, micropowder) was provided by Fluidinova (Moreira da Maia, Portugal), glutaraldehyde (GA; 50% aqueous solution) by Scharlau (Barcelona, Spain) and hydrogen peroxide (H2O2; 30% aqueous solution) by Honeywell Fluka (Madrid, Spain). CO2 (purity > 99.9%) was supplied by Nippon Gases (Madrid, Spain) and absolute ethanol (EtOH) by VWR (Radnor, PA, USA). Water was purified using reverse osmosis (resistivity > 18 MΩ·cm; Milli-Q, Millipore®, Madrid, Spain).
5
Antioxidant Capacity Determination Protocol
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Sodium carbonate (Na2CO3), sodium hydrogen phosphate (Na2HPO4), and potassium dihydrogen phosphate (KH2PO4) were acquired from VWR (Saint-Prix, France). Nitric acid (HNO3) was obtained from Panreac (Barcelona, Spain). Methanol, absolute ethanol (EtOH) and hexane were purchased from VWR Chemicals (Rosny-sous-Bois, France). CO2 was obtained from Carburos Metálicos (Massalfassar, Valencia, Spain). EtOH 96° was obtained from Guinama (La Pobla de Vallbona, Valencia, España). Folin–Ciocalteu reagent, potassium persulfate (K2S2O8), gallic acid, ABTS (2,2-Azino-Bis-3-eThylbenzothiazoline-6-Sulphonic Acid), fluorescein, 2,2-Azobis(2-amidinopropane) dihydrochloride (AAPH), and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were purchased from Sigma Aldrich (Steinheim, Baden-Württemberg, Germany).
6
Synthesis and Characterization of Chitosan-Based CO2 Sorbents
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Chitosan (deacetylation degree 75–85%, viscosity 20–300 mPa·s, Mw 50–190 kDa), glutaraldehyde solution (grade II, 25 wt.% in H2O), 4-vinylbenzyl chloride (90% purity), poly(diallyldimethylammonium chloride) (P[DADMA]Cl) solution (20 wt. % in H2O, viscosity 250–500 mPa·s, average Mw 200,000–350,000 medium molecular weight), 1-methylpyrrolidine (≥ 98.0% purity), triethylamine (≥ 99.5% purity), and anion exchange resin Amberlyst A-26 (OH- form) were supplied by Sigma-Aldrich (St. Louis, MO, USA). 2,2′-Azobis(2-methylpropionitrile) (AIBN) was purchased from Glentham Life Sciences (Corsham, UK). Glacial acetic acid and absolute ethanol (EtOH) were both purchased from VWR (Radnor, PA, USA). NaOH (98% purity) was purchased from Panreac (Barcelona, Spain). DMSO-d6 was purchased from Euriso-top (Saint-Aubin, France). Water was purified using reverse osmosis (resistivity > 18 MΩ.cm, Milli-Q, Millipore®, Madrid, Spain). Carbon dioxide (99.8% purity) was supplied by Nippon Gases (Madrid, Spain). He 4.6 and CO2 4.5, supplied by Linde Portugal, were used in thermogravimetric analysis (TGA) and CO2 capture experiments. All chemicals were used without further purification.
7
Sterilization of Biomaterials Using CO2 and Ethanol
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Native corn starch (52.6% amylose content) was provided by Roquette Frères S.A. (Lestrem, France). Zein (m.p. 266–283 °C, size of dry agglomerates by the sieving method: 557 ± 208 µm) was purchased from Sigma-Aldrich, Inc. (Madrid, Spain). CO2 (purity of >99.9%) was supplied by Praxair, Inc. (Madrid, Spain). Sterilization reel were purchased from E-line S.r.l. (Torre Pallavicina, Italy). Commercial spore strips with 106 spores of Bacillus stearothermophilus (ATCC 7953) and Bacillus pumilus (ATCC 27142) were purchased from Sigma-Aldrich, Inc. (Madrid, Spain) and Bacillus atrophaeus (cell line 9372) spores were obtained from Crosstex International, Inc. (Rush, NY, USA). Absolute ethanol (EtOH) was provided by VWR (Radnor, PA, USA). T. Trypticase soy broth (TSB) medium was purchased from BIOKAR Diagnosis (Pantin, France) and hydrogen peroxide 30% (v/v) from Sigma-Aldrich, Inc. (Madrid, Spain). Human bone marrow-derived mesenchymal stem cells (ATCC® PCS-500-012™) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Minimum Essential Medium Alpha (αMEM) and Opti-MEMTM were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum and penicillin 10,000 U/mL–streptomycin 10 mg/mL were supplied by Sigma-Aldrich (Saint Louis, MO, USA). Cell proliferation reagent WST-1 was supplied by Roche (Basel, Switzerland).
8
Synthesis and Characterization of Rare-Earth Doped Silica Nanoparticles
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Ytterbium (III) oxide (Yb2O3, 99.9% purity), erbium (III) oxide (Er2O3, 99.9%), ytterbium (III) nitrate pentahydrate (Yb(NO3)3·5H2O, 99.9%), yttrium(III) nitrate hexahydrate (Y(NO3)3·6H2O, 99.8%), sodium fluoride (NaF, >99%) and ethylenediaminetetraacetic acid (EDTA) were provided by Sigma Aldrich (Merck Group, Darmstadt, Germany). Yttrium (III) oxide (Y2O3, 99.99%), erbium (III) nitrate pentahydrate (Er(NO3)3·5H2O, 99.9%), and tetraethyl orthosilicate (TEOS, Si(OC2H5)4, 98%), were obtained from Acros Organics (Thermo-Fisher Scientific, Geel, Belgium). Hydrochloric acid (HCl, 37%) was supplied by Fisher (Madrid, Spain) and ammonium hydroxide (25% NH3 in H2O) by Fluka Analytical (Honeywell International, Charlotte, NC, USA). CO2 (purity > 99.9%) was obtained from Nippon Gases (Madrid, Spain) and absolute ethanol (EtOH) from VWR (Radnor, PA, USA). All reagents were used as purchased and deionized water was employed in all the procedures.
9
Graphene Nanoparticle Synthesis and Characterization
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GNPs (grade Micrograf HC11) were provided by Nacional de Grafite, Brazil; IDA (purity ≥ 98%), PFA, NaHCO3, NaOH, and potassium hydrogen phthalate (KHP), of reagent grade, were purchased from Sigma Aldrich; absolute ethanol (EtOH) was purchased from VWR Chemicals; acetone and Na2CO3, of analytical reagent grade, were purchased from Fisher Scientific; hydrochloric acid (HCl, fuming, 37%) was purchased from Honeywell Fluka. All chemicals/reagents were used as received.
10
Functionalized Silica Nanoparticles for VEGF Delivery
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Methanol,
tetraethyl orthosilicate (TEOS,
≥99.0%), triethyl phosphate (TEP, ≥99.8%), calcium nitrate
tetrahydrate (CNT, ≥98%), 3-aminopropyl triethoxysilane (APTES),
albumin–fluorescein isothiocyanate conjugate (FITC albumin), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (sulfo-NHS), albumin–fluorescein isothiocyanate conjugate
(albuminFITC), and 37% paraformaldehyde (PFA) were all
obtained from Sigma Aldrich. Aqueous ammonia (25%) was purchased form
Carl Roth. Absolute ethanol (EtOH) and phosphate-buffered saline (tablets,
PBS) were purchased from VWR. NHS–Fluorescein (5/6-carboxyfluorescein
succinimidyl ester) was purchased from Thermo Fisher. Human vascular
endothelial growth factor (VEGF165) was purchased from
Miltenyi.
tetraethyl orthosilicate (TEOS,
≥99.0%), triethyl phosphate (TEP, ≥99.8%), calcium nitrate
tetrahydrate (CNT, ≥98%), 3-aminopropyl triethoxysilane (APTES),
albumin–fluorescein isothiocyanate conjugate (FITC albumin), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (sulfo-NHS), albumin–fluorescein isothiocyanate conjugate
(albuminFITC), and 37% paraformaldehyde (PFA) were all
obtained from Sigma Aldrich. Aqueous ammonia (25%) was purchased form
Carl Roth. Absolute ethanol (EtOH) and phosphate-buffered saline (tablets,
PBS) were purchased from VWR. NHS–Fluorescein (5/6-carboxyfluorescein
succinimidyl ester) was purchased from Thermo Fisher. Human vascular
endothelial growth factor (VEGF165) was purchased from
Miltenyi.
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