Jem 100cx

The FANP were prepared by an emulsion/solvent evaporation method39 (link)40 (link). In brief, 25 mg of MPEG-PCL, 2 mg of FA-PEG-PCL and 3 mg of FITC-PEG-PCL were dissolved in 1 mL of dichloromethane and then added to 5 mL of 0.6% sodium cholate hydrate solution. Subsequently, the solution was sonicated by a probe sonicator at 200 W for 150 s on ice. Then dichloromethane was removed by rotary evaporation and the FITC loaded FANP was condensed to a fixed concentration by ultrafiltration at 4000 g. To prepare DiR loaded FANP, 3 mg FITC-PEG-PCL was changed to 3 mg MPEG-PCL and 600 μg DiR was added, and then prepared as above. After changed FA-PEG-PCL to MPEG-PCL, the FITC loaded NP and DiR loaded NP could be obtained using above described procedures.
The mean particle sizes and zeta potentials of FANP and NP were determined by Malvern Zetasizer Nano ZS90 instrument (Malvern Instrument Ltd., UK). The morphology of FANP and NP was captured by transmission electron microscope (TEM) (JEM-100CX, JEOL, Japan).

Virus-containing allantoic fluids were centrifuged at 3000 RPM for 30 min. The supernatant was separated and then subjected to ultracentrifugation at 52500g for 40 min. The resulting pellets were diluted in a minimal volume of 0.05 M phosphate buffer. Virus specimens were prepared on formvar film-coated grids with the addition of a layer of evaporated carbon. The specimens were contrasted with 3% phosphotungstic acid solution and then examined in an electron microscope JEM 100 CX (JEOL, Japan).