Cell surface markers were characterized in the SVF cell population by flow cytometry using a LSR II flow cytometer (BD BioSciences). The SVF cells were freshly isolated, filtered through a 100 um screen to remove large debris, and cell markers for VEGFR2, c-Kit (Abcam), CD11b (eBioscience), CD31 (BD BioSciences), CXCR2 (R&D Systems), CD47, CD34, and PDGFR (Santa Cruz) were used. Secondary antibodies, when appropriate, were used (goat-anti-rabbit and Streptavidin, Abcam). Antibody dilutions were optimized for each marker. Single color tubes were used to generate compensation controls. Tubes containing unstained SVF and additional tubes treated with appropriate isotype controls were used in setting discrimination gates. Positive populations were identified by quadrant gating dot plots using FlowJo 7.6.2 analysis software (Tree Star Inc, Ashland, OR).
Pdgfr
Manufactured by Santa Cruz Biotechnology
Sourced in United States
PDGFR (Platelet-Derived Growth Factor Receptor) is a tyrosine kinase receptor that binds to and is activated by platelet-derived growth factors (PDGF). It plays a crucial role in cellular processes such as cell proliferation, survival, migration, and differentiation.
Automatically generated - may contain errors
Lab products found in correlation
Cxcr2, by R&D Systems (1 mentions)
Cd11b, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Anti mouse and anti rabbit igg conjugated horseradish peroxidase, by Santa Cruz Biotechnology (1 mentions)
Recombinant human bfgf, by R&D Systems (1 mentions)
P c src, by Santa Cruz Biotechnology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
C src, by Santa Cruz Biotechnology (1 mentions)
Mir 381 mimic, by Thermo Fisher Scientific (1 mentions)
Vegf c antibody, by Abcam (1 mentions)
Control mirna, by Thermo Fisher Scientific (1 mentions)
Protein a g bead, by Santa Cruz Biotechnology (1 mentions)
F12 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
2 protocols using pdgfr
1
Multiparametric Characterization of SVF Cells
2
Molecular Pathways Investigation Toolkit
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein A/G beads; anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase; rabbit polyclonal antibodies specific for p-PDGFR, PDGFR, p-c-Src, and c-Src were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant human bFGF was purchased from R&D Systems (Minneapolis, MN, USA). VEGF-C antibody was purchased from Abcam (Cambridge, MA, USA). Dulbecco's modified Eagle's medium (DMEM), F-12 medium, fetal bovine serum (FBS) and all other cell culture reagents were purchased from Gibco-BRL Life Technologies (Grand Island, NY, USA). ON-TARGETplus siRNAs were purchased from Dharmacon Research (Lafayette, CO, USA). miR-381 mimic, miRNA control, Lipofectamine 2000, and Trizol were purchased from Life Technologies (Carlsbad, CA, USA). All other chemicals were sourced from Sigma-Aldrich (St Louis, MO, USA).
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