PD-L1-specific reporter plasmid, pGL3 1 kb promoter was received from Addgene (Watertown, MT, USA). Cancer cells were transfected with reporter plasmid using Lipofectamine 3000 (Invitrogen). PD-L1 reporter gene was analyzed with a luciferase assay system (Promega, Madison, WI, USA) and a luminometer. β-Galactosidase reporter gene was analyzed with Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System (Invitrogen) and has been experimented with for use as an internal control.
Galacto light plus β galactosidase reporter gene assay system
Manufactured by Thermo Fisher Scientific
Sourced in Switzerland
The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is a chemiluminescent reporter gene assay used to detect and quantify β-galactosidase activity in biological samples. It provides a sensitive and convenient method for measuring gene expression levels.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using galacto light plus β galactosidase reporter gene assay system
1
PD-L1 Reporter Assay Protocol
2
HEK293 Dual Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293 cells were plated in 6 well plate and transfected with 1 µg of pNeuLite (Addgene) alone or in combination with 0.5 µg of pcDNA3.1-flag-ELF3WT (provided from Dr. Seung Bae Rho, National Cancer Center, Republic of Korea) and p3Xflag-myc-CMV26-MED23 mutant plasmids as indicated. For the normalization of the luciferase activity of each sample, 0.5 µg of β-galactosidase expression plasmid (provided from Dr. Eun-Sook Hwang, Ewha Womans University, Republic of Korea) was also added in every sample groups. All of the transfections were conducted using Lipofectamine® 2000 Transfection Reagent (Invitrogen, USA). After 24 h, firefly luciferase and β-galactosidase activities were evaluated with the Infinite M200 PRO Microplate reader (Tecan Group ltd., Switzerland) using the Luciferase Assay System (Promega) and Galacto-Light Plus β-Galactosidase Reporter Gene Assay System (Invitrogen), respectively, according to the manufacturers’ protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!