Pe anti mouse cd8a ly 2 53 6.7

Manufactured by BD

PE anti-mouse CD8a (Ly-2)(53-6.7) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8a (Ly-2) antigen expressed on the surface of mouse cytotoxic T lymphocytes. This product is intended for use in flow cytometry applications to identify and quantify CD8+ T cells.

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Lab products found in correlation

Foxp3 apc fjk 16s, by Thermo Fisher Scientific (2 mentions) Anti cd3 apc cy7 clone 17a2, by BD (2 mentions) Anti mouse cd69 fitc clone h1.2f3, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

CD8a-PE (22 citations) Anti-CD8a (20 citations) CD8a (53-6.7, (18 citations) Anti-CD8a-PE (10 citations) PE anti-mouse CD8a (8 citations) Anti-CD8a (Ly-2) (3 citations) Anti-CD8a (53-6.7) (2 citations) Ly-6-PE (2 citations) Mouse anti (2 citations)

2 protocols using pe anti mouse cd8a ly 2 53 6.7

T Cell Flow Cytometry Panel Protocol

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The T cell flow cytometry panel included the following directly conjugated primary antibodies: anti-mouse CD45 superbright 600 clone: 30-511 (ref# 63-0451-82, eBioscience), anti-CD3 APC-Cy7 clone 17A2(BD Biosciences, cat # 560590), eBioscience anti-mouse CD4 PE-Cy7 clone: RM4-5 (Ref # 25-0042-82, Invitrogen), PE anti-mouse CD8a (Ly-2)(53-6.7) (cat # 553032, BD), anti-mouse CD69 FITC clone: H1.2F3 (Ref# 11-0691-81, eBioscience), and Foxp3 (FJK-16s) APC (eBioscience). Gating strategies are as follows:
CD4+ T cell: live/CD45+/CD3+/CD4+/Foxp3-
CD8+ T cell: live/CD45+/CD3+/CD8+
Treg: live/CD45+/CD3+/CD4+/Foxp3+
Activated CD8+ T cell: live/CD45+/CD3+/CD8+/CD69+

Huntington K.E., Louie A.D., Srinivasan P.R., Schorl C., Lu S., Silverberg D., Newhouse D., Wu Z., Zhou L., Borden B.A., Giles F.J., Dooner M., Carneiro B.A, & El-Deiry W.S. (2023). GSK-3 Inhibitor Elraglusib Enhances Tumor-Infiltrating Immune Cell Activation in Tumor Biopsies and Synergizes with Anti-PD-L1 in a Murine Model of Colorectal Cancer. International Journal of Molecular Sciences, 24(13), 10870.

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Comprehensive T Cell Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The T cell flow cytometry panel included the following directly-conjugated primary antibodies: Anti-mouse CD45 superbright 600 clone: 30–511 (ref# 63-0451-82, eBioscience), anti-CD3 APC-Cy7 clone 17A2(BD Biosciences, cat # 560590), eBioscience anti-mouse CD4 PE-Cy7 clone: RM4–5 (Ref # 25-0042-82, Invitrogen), PE anti-mouse CD8a (Ly-2)(53–6.7) (cat # 553032, BD), Anti-mouse CD69 FITC clone: H1.2F3 (Ref# 11-0691-81, eBioscience), and Foxp3 (FJK-16s) APC (eBioscience). Gating strategies are as follows:
CD4+ T cell: live/CD45+/CD3+/CD4+/Foxp3−
CD8+ T cell: live/CD45+/CD3+/CD8+
Treg: live/CD45+/CD3+/CD4+/Foxp3+
Activated CD8+ T cell: live/CD45+/CD3+/CD8+/CD69+

Huntington K.E., Louie A.D., Srinivasan P.R., Schorl C., Lu S., Silverberg D., Newhouse D., Wu Z., Zhou L., Borden B.A., Giles F.J., Dooner M., Carneiro B.A, & El-Deiry W.S. (2023). GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancer. bioRxiv.

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