J 1000 cd spectrometer

Manufactured by Jasco

Sourced in United States

The J-1000 CD spectrometer is a laboratory instrument used for measuring the circular dichroism (CD) of samples. It is designed to analyze the absorption of left and right circularly polarized light by a sample, providing information about the structural properties of molecules such as proteins, nucleic acids, and other chiral compounds.

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Thermo grams suite software, by Thermo Fisher Scientific (1 mentions)

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CD spectrometer (22 citations) J-1000 (2 citations)

7 protocols using j 1000 cd spectrometer

Circular Dichroism Spectroscopy Analysis

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After 48 h of TTR incubation
at 37 °C under constant agitation at 510 rpm, samples were diluted
to the final concentration of 100 μM using PBS and measured
immediately using a Jasco J1000 CD spectrometer (Jasco, Easton, MD).
Three spectra were collected for each sample within 190–250
nm and averaged.

Ali A., Zhaliazka K., Dou T., Holman A.P, & Kurouski D. (2023). Role of Saturation and Length of Fatty Acids of Phosphatidylserine in the Aggregation of Transthyretin. ACS Chemical Neuroscience, 14(18), 3499-3506.

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Circular Dichroism Spectroscopy Assay

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After 5 and 24 h of sample
incubation, the samples were diluted to the final concentration of
100 μM using PBS and measured immediately using a J-1000 CD
spectrometer (Jasco, Easton, MD). Three spectra were collected for
each sample within 205–250 nm.

Joshi R., Zhaliazka K., Holman A.P, & Kurouski D. (2023). Elucidation of the Role of Lipids in Late Endosomes on the Aggregation of Insulin. ACS Chemical Neuroscience, 14(18), 3551-3559.

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Circular Dichroism Spectroscopy

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After 24 h of sample incubation, samples were diluted to the final concentration of 100 μM using PBS and measured immediately using J-1000 CD spectrometer (Jasco, Easton, MD, USA). Three spectra were collected for each sample within 205–250 nm.

Matveyenka M., Rizevsky S., Pellois J.P, & Kurouski D. (2022). Lipids uniquely alter rates of insulin aggregation and lower toxicity of amyloid aggregates. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1868(1), 159247.

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CD Spectroscopy of Samples

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CD spectra were collected from all samples at 25 ºC using a J-1000 CD spectrometer (Jasco, Easton, MD, USA). For each sample, three spectra were collected within 205–250 nm.

Sullivan J.T., Woodruff M., Lettieri J., Agarwal V., Krol G.J., Leese P.T., Watson S, & Heller A.H. (1999). Pharmacokinetics of a Once-Daily Oral Dose of Moxifloxacin (Bay 12-8039), a New Enantiomerically Pure 8-Methoxy Quinolone. Antimicrobial Agents and Chemotherapy, 43(11), 2793-2797.

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Protein Aggregation CD Spectroscopy

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For all measurements, 300 μL
of the protein aggregate was collected and added into a 1 mm quartz
cuvette. CD spectra were acquired on a J-1000 CD spectrometer (Jasco,
Easton, MD, USA). Three spectra were collected for each sample within
195–250 nm and averaged using Thermo Grams Suite software (Thermo
Fisher Scientific, Waltham, MA, USA).

Hoover Z., Lynn M., Zhaliazka K., Holman A.P., Dou T, & Kurouski D. (2023). Long-Chain Polyunsaturated Fatty Acids Accelerate the Rate of Insulin Aggregation and Enhance Toxicity of Insulin Aggregates. ACS Chemical Neuroscience, 15(1), 147-154.

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Circular Dichroism of α-Synuclein

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After 146 h of incubation at
37 °C, α-syn, α-syn:PA-C_18:1, α-syn:PA-C_16:0, and α-syn:PA-C_18:0 were diluted to the
final concentration of 100 μM using PBS and measured immediately
using a Jasco J1000 CD spectrometer (Jasco, Easton, MD). Three spectra
were collected for each sample within 190–250 nm and averaged.

Ali A., Holman A.P., Rodriguez A., Zhaliazka K., Osborne L, & Kurouski D. (2024). Large Unilamellar Vesicles of Phosphatidic Acid Reduce the Toxicity of α-Synuclein Fibrils. Molecular Pharmaceutics, 21(3), 1334-1341.

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CD Spectroscopy of Amyloid-Beta

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CD spectra were collected from 100 μM solution of Aβ_1–42 in 20 mM phosphate buffer, pH 7.4, at 25 °C on a J-1000 CD spectrometer (Jasco, Easton, MD, USA). Three spectra were collected for each sample within 205–250 nm.

Zhaliazka K, & Kurouski D. (2022). Nanoscale Characterization of Parallel and Antiparallel β-Sheet Amyloid Beta 1–42 Aggregates. ACS chemical neuroscience, 13(19), 2813-2820.

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