Bx43 light microscopy

Synovial sarcoma specimensThis study was conducted as a preliminary experiment to test the efficacy of two FISH protocols carried out at Hospital Universiti Sains Malaysia from a period of 12 months from July 2016 to July 2017. Ethical clearance for the study was obtained from the Ethical Committee, Universiti Sains Malaysia (JEPEM Code: USM/JEPeM/16050189). A total of 27 cases diagnosed histologically as synovial sarcoma (SS), were retrieved from registry book and computerized registry data files were selected. Representative paraffin block of the tumors was utilized in this study.
Histology assessment The tissue slides were deparaffinized and hydrated using standard procedures and stained with hematoxylin and eosin (H&E). The slides were viewed using Olympus BX43 light microscopy to identify the optimal region of the tumor.
Fluorescent in situ hybridization (FISH)The Cytocell FISH protocol was carried out based on the Cytocell SYT Breakapart probe protocol (Cytocell, United Kingdom) as instructed in the user manual pamphlet. The Optimised Dako FISH protocol was conducted using modified Dako Histology FISH Accessory Kit (Agilent, United States). The SYT-SSX break apart probe from Cytocell was used in both Cytocell FISH protocol and Optimised Dako FISH protocol. The procedure for Cytocell FISH protocol and Optimised Dako FISH protocol is summarized in Table 1.

After 2-weeks culturing, ALI culture inserts were fixed with 4% paraformaldehyde, and dehydrated in gradient alcohol series before they were embedded in paraffin. Sections of 4-μm thickness were employed for hematoxylin and eosin (HE) staining. The morphology of cells was observed under the Olympus BX43 light microscopy equipped with DP-73 camera (Olympus China, Shanghai, China).