4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid (C75) (sc-202511), 5-Tetradecyloxy-2-furonic acid (TOFA) (sc-200653), and etomoxir (sc-208284) were purchased from Santa Cruz Biotechnology. Trimetazidine (TMZ) (S61054) was purchased from Shyuanye Biotechnology. High glucose dulbecco's modified eagle medium (DMEM) (11995040), low glucose DMEM (11885084), no glucose DMEM (11885084), high glucose DMEM with no glutamine (11960069), RPMI 1640 medium (21875091), glucose free RPMI 1640 medium (11879020), and glutamine free RPMI 1640 medium (21870076) were obtained from Gibco. The following primary antibodies were used in the study: rabbit monoclonal anti-MDA5 (Sigma-Aldrich, SAB2101127), rabbit polyclonal anti-RIG-I (Cell Signaling Technology, 3743), rabbit polyclonal anti-NF-κB (Beyotime, AF0246), rabbit monoclonal anti-Phospho-IRF3 (Beyotime, AF1594), and mouse monoclonal anti-GAPDH (Beyotime, AG019) and mouse monoclonal anti-CSFV E2 (WH303) (JBT, 9011). Mouse polyclonal anti-CSFV Npro was kindly provided by Dr. Xinglong Yu (Veterinary Department, Hunan Agricultural University, China). Te secondary antibodies were used in the study: HRP-conjugated goat anti-mouse IgG (Beyotime, A0192), HRP-conjugated goat anti-rabbit IgG (Beyotime, A0208) and Alexa Fluor 488-labeled Goat Anti-Mouse IgG (Beyotime, A0428).
Free rpmi 1640 medium
Manufactured by Thermo Fisher Scientific
Sourced in China
RPMI 1640 medium is a widely used cell culture medium developed for the growth of human cells. It provides a balanced salt solution and essential nutrients required for cell proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Attune nxt, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle medium dmem high glucose, by Thermo Fisher Scientific (1 mentions)
Dmem no glucose, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 labeled goat anti mouse igg, by Beyotime (1 mentions)
Hrp conjugated goat anti mouse igg, by Beyotime (1 mentions)
Rpmi 1640 glucose free medium, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated goat anti rabbit igg, by Beyotime (1 mentions)
Dchf da, by Merck Group (1 mentions)
N acetyl cysteine (nac), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
2 protocols using free rpmi 1640 medium
1
Comparative Analysis of Antiviral Drug Compounds
2
Reactive Oxygen Species Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For ROS determination, 2 × 106 BMDCs were seeded
on 6-well plates overnight and then treated with or without Ag NPs
(1 μg/mL) and N-acetylcysteine (NAC, 5 mM,
Sigma) for 24 h. Then, cells were washed 3 times with cold PBS (Solarbio,
China) and incubated with 10 μM DCHF-DA (Sigma) in serum-free
RPMI 1640 medium (Gibco) at 37 °C for another 30 min. Cells were
then washed with cold PBS (Solarbio, China) 3 times, collected, and
measured by flow cytometry (Attune NxT, Thermo Fisher Scientific).
on 6-well plates overnight and then treated with or without Ag NPs
(1 μg/mL) and N-acetylcysteine (NAC, 5 mM,
Sigma) for 24 h. Then, cells were washed 3 times with cold PBS (Solarbio,
China) and incubated with 10 μM DCHF-DA (Sigma) in serum-free
RPMI 1640 medium (Gibco) at 37 °C for another 30 min. Cells were
then washed with cold PBS (Solarbio, China) 3 times, collected, and
measured by flow cytometry (Attune NxT, Thermo Fisher Scientific).
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