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Tiazolyl blue tetrazolium bromide

Manufactured by Merck Group

Tiazolyl blue tetrazolium bromide is a colorimetric reagent commonly used in cell viability assays. It functions as a dye that is reduced by metabolically active cells, producing a purple-colored formazan product that can be quantified to measure cellular activity.

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2 protocols using tiazolyl blue tetrazolium bromide

1

Evaluation of Paclitaxel Formulations with Cyclodextrins

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Paclitaxel was purchased from Sigma Aldrich (St. Louis, MO). Hydroxypropyl β-cyclodextrin (HPCD, Kleptose®) and Methyl β-cyclodextrin (MeCD, Crysmeb) were purchased from Roquette Pharma (Lestrem, France). Sulfobutyl ether β-cyclodextrin (SBCD) was purchased from Cydex Pharmaceuticals (Lenexa, KS). Tert-butyl alcohol was purchased from Alfa Aesar (Ward Hill, MA). Cyclophosphamide (CP), Ethylmethyl sulfone (EMS), Glucose 6-phosphate, Acetonitrile, Methanol Dimethyl Sulfoxide (DMSO), Tween 20, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), Tiazolyl blue tetrazolium bromide, (MTT), Sodium dodecyl sulfate (SDS) and Dimethyl formamide (DMF) were purchased from Sigma Aldrich (St. Louis, MO). Nicotinamide adenine dinucleotide phosphate (NADP) was obtained from Fisher Chemicals (Suwannee, GA).
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2

Carvacrol Oil Cytotoxicity Evaluation

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Carvacrol oil (5-isopropryl-2-methylphenol, purity 98%), sorbitan monostearate 80 (Span 80®), and Tiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Polysorbate 80 (Tween 80®) was provided by Vetec Química Fina LTDA (Duque de Caxias, RJ, Brazil). Medium chain triglyceride (Mygliol 812®) was acquired from Sasol (Brunsbüttel, Germany). RPMI 1,640 culture medium Dulbecco’s Modifed Eagle Medium (DMEM), inactivated fetal bovine serum and antibiotics (10,000 units/ml Penicillin G sodium salt and 10 mg/ml streptomycin sulfate) were purchased from Vitrocell (Campinas, SP, Brazil). Praziquantel was kindly provided by Ecovet Indústria Veterinária Ltda (São Paulo, Brazil).
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