The DU-145, LNCaP, T47D and SKBR3 cell lines were purchased from DSMZ (Braunschweig, Germany), and were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), at 37 °C, 5% CO2. All media were purchased from Invitrogen (Carlsbad, USA) and all chemicals from Sigma (St. Louis, MO), unless otherwise stated.
Fetal bovine serum (fbs)
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
Fetal bovine serum (FBS) is a commonly used cell culture supplement derived from the blood of bovine fetuses. It contains a complex mixture of proteins, growth factors, and other nutrients that support the growth and proliferation of cells in vitro. FBS is a core component in the maintenance and expansion of various cell lines, including mammalian, insect, and microbial cultures.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Du145, by Leibniz Institute DSMZ (1 mentions)
Lncap, by Leibniz Institute DSMZ (1 mentions)
Sk br 3, by Leibniz Institute DSMZ (1 mentions)
Recombinant il 2, by Thermo Fisher Scientific (1 mentions)
Penicillin streptavidin, by Merck Group (1 mentions)
Mycospy master mix, by Biontex (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Oci ly1, by Leibniz Institute DSMZ (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Mycoplasma detection kit, by Lonza (1 mentions)
Dulbecco modified eagle medium (dmem), by Leibniz Institute DSMZ (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sk n mc, by American Type Culture Collection (1 mentions)
Ntera 2, by Leibniz Institute DSMZ (1 mentions)
Sup b15, by Leibniz Institute DSMZ (1 mentions)
4 protocols using fetal bovine serum (fbs)
1
Cell Culture of Cancer Cell Lines
2
Cultivation of Human Cell Lines
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The human cancer cell lines A2780 (ECACC #93112519), A2780Ccis (ECACC # 93112517), A549 (ATCC-CCL-185), HT29 (ATCC-HTB-38), and MCF7 (ATCC-HTB-22) were cultivated in RPMI-1640 medium. Non-malignant human fibroblasts CCD18Co (ATCC-CRL-1459) were grown in MEME (both from Sigma-Aldrich, St. Louis, MO, USA). Both media were supplemented with 10% fetal bovine serum (Biowest, Nuaillé, France) and 1% penicillin-streptomycin (Sigma-Aldrich).
The cell lines HH (Cat# ACC 707; RRID:CVCL_1280), and HG-3 (Cat# ACC 765; RRID:CVCL_Y547) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and cultivated in RPMI-1640 Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptavidin (P0781, Sigma-Aldrich, St. Louis, MO). DERL-2 (Cat# ACC 531; RRID:CVCL_2016) was obtained from DSMZ and cultivated in RPMI-1640 Medium with 20% fetal bovine serum, 1% penicillin/streptavidin, and 0.02 ng/µL recombinant IL-2 (200-02, Peprotech, Hamburg, Germany). Oci-Ly1 (Cat# ACC 722; RRID:CVCL_1879) was obtained from DSMZ and cultivated in RPMI-1640 Medium with 20% fetal bovine serum and 1% penicillin/streptavidin.
All cell lines were cultured in a humidified atmosphere at 37 °C and 5% CO2. Passaging did not exceed ten times after thawing. Mycoplasma negativity was confirmed repeatedly using MycoSPY® Master Mix (M020, Biontex, Munich, Germany).
The cell lines HH (Cat# ACC 707; RRID:CVCL_1280), and HG-3 (Cat# ACC 765; RRID:CVCL_Y547) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and cultivated in RPMI-1640 Medium supplemented with 10% fetal bovine serum and 1% penicillin/streptavidin (P0781, Sigma-Aldrich, St. Louis, MO). DERL-2 (Cat# ACC 531; RRID:CVCL_2016) was obtained from DSMZ and cultivated in RPMI-1640 Medium with 20% fetal bovine serum, 1% penicillin/streptavidin, and 0.02 ng/µL recombinant IL-2 (200-02, Peprotech, Hamburg, Germany). Oci-Ly1 (Cat# ACC 722; RRID:CVCL_1879) was obtained from DSMZ and cultivated in RPMI-1640 Medium with 20% fetal bovine serum and 1% penicillin/streptavidin.
All cell lines were cultured in a humidified atmosphere at 37 °C and 5% CO2. Passaging did not exceed ten times after thawing. Mycoplasma negativity was confirmed repeatedly using MycoSPY® Master Mix (M020, Biontex, Munich, Germany).
3
Characterization of Mutant p53 Pancreatic Cancer Cell Lines
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Homozygous mutant human pancreatic cancer cell lines MIA-PACA-2 (mutp53R248W) (DZMS, RRID : CVCL_0428), PANC-1 (mutp53R273H) (ATCC, RRID : CVCL_0480), BXPC-3 (mutp53Y220C) (ATCC, RRID : CVCL_0186), and PA-TU-8902 (mutp53C176S) (DSMZ, RRID : CVCL_1845) were grown in DMEM (Gibco) with 10% FBS (Merck). PA-TU-8988T (mutp53R282W) (DSMZ, RRID : CVCL_1847) were grown in DMEM medium with 5% FBS. CAPAN-1 (mutp53A159V) (ATCC, RRID : CVCL_0237) were grown in RPMI 1640 (Gibco) with 20% FBS, and L3.6pl cells (truncating frameshift p53 mutation) (37 (link), 38 (link)) were grown in RPMI 1640 with 10% FBS. All media were supplemented with Penicillin-Streptomycin (10,000 U/mL, Gibco) and L-Glutamine (Gibco). All cell lines were grown at 37°C at 5% CO2 in a humidified atmosphere and tested for mycoplasma contamination on a regular basis (Mycoplasma Detection Kit, Lonza). Cell line authentication certificates are provided as Supplemental Material
4
Ewing Sarcoma Cell Line Protocol
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EwS cell lines were grown as adherent cultures on collagen-coated flasks in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (Thermo Fisher, Dreieich, Germany) and 2 mM L-glutamin (Sigma-Aldrich, Taufkirchen, Germany) and maintained at 37 °C and 5% CO2. TC-32, TTC-466, 5838, A4573 were gifts from the Children’s Hospital Los Angeles, United States. CADO-ES-1 (#ACC 255), RD-ES (#ACC 260), TC-71 (#ACC 516) and NTERA-2 (#ACC 527) were purchased from DSMZ (Braunschweig, Germany). A673 (#CRL-1598) and SK-N-MC (#HTB-10) from ATCC (Manassas, Virginia, USA). Cell lines MS-EwS-6, MS-EwS-15 and MS-EwS-16 were established in our institution, as reported previously31 (link), and WE-68 and VH-64 were gifts from the Institute of Experimental Orthopedics of our institution. Control cell lines SUP-B15 (B lineage leukemia) (#ACC 389) and HT-1080 (fibrosarcoma) (#ACC 315) were purchased from DSMZ and were cultivated in RMPI 1640 medium supplemented with 10% heat-inactivated FBS and 2 mM l -glutamin. Short tandem repeat (STR) profiling was used to confirm the identity of all cell lines (Supplementary Table 1 ), and all cell lines were regularly checked by PCR to exclude mycobacterial contamination.
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