Mouse monoclonal β actin antibody

PLC/PRF/5 and Hep3B cells were grown in complete MEM medium with 10% FBS. 5-FU was obtained from Sigma-Aldrich (St. Louis, MO, USA). The PCAF-expressing plasmid and its empty plasmid pCMV6-Entry were both from Origene Technologies Inc. (Rockville, MD, USA). The cDNA of GLI1 was cloned into the pCMV-Tag2B vector from Stratagene (Santa Clara, CA, USA) as GLI1-expressing plasmid. The rabbit monoclonal PCAF antibody, mouse monoclonal GLI1 antibody, mouse monoclonal PTCH1 antibody, rabbit monoclonal Bcl-2 antibody and rabbit monoclonal BAX antibody were from Cell Signaling (Danvers, MA, USA). The rabbit polyclonal anti-acetyl lysine antibody was obtained from Abcam (Cambridge, MA, USA). The mouse monoclonal β-actin antibody was from Boster Biotechnology (Wuhan, China). The IHC detection kit (Catalog No.: SP-9001) was purchased from ZSGB Bio. (Beijing, China). Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit was from Invitrogen (Carlsbad, CA, USA).

DMEM medium, RPMI 1640 medium, FBS and trypsin/EDTA were purchased from Invitrogen Co. (Carlsbad, CA, USA). The GLI1 expressing plasmid was constructed by cloning GLI1 cDNA into the pCMV-Tag2B vector from Stratagene (Santa Clara, CA). Cav-1 cDNA was cloned into the pCMV-Tag2B vector to construct Cav-1 expressing plasmid. Both GLI1 siRNAs (Catalog No.: sc-37911) and Cav-1 siRNAs (Catalog No.: sc-29241) were from Santa Cruz Biotechnology, Santa Cruz, CA, USA). The 18s rRNA TaqMan probe (Hs99999901_s1), Cav-1 TaqMan probe (Hs00971716_m1) and GLI1 TaqMan probe (Hs01110766_m1) were purchased from Applied Biosystems (Carlsbad, CA, USA). The rabbit polyclonal GLI1 antibody, rabbit polyclonal Cav-1 antibody, rabbit polyclonal SNAI1 antibody, rabbit polyclonal Slug antibody and rabbit polyclonal PTCH1 antibody were from Cell signaling (Danvers, MA, USA). The mouse monoclonal E-cadherin antibody and rabbit polyclonal N-cadherin antibody were both purchased from Santa Cruz Biotechnology. The rabbit polyclonal Twist antibody was from Abcam (MA, USA). The rabbit polyclonal Fibronectin antibody, mouse monoclonal Vimentin antibody, mouse monoclonal β-actin antibody and 4,6-diamidino-2-phenylindole (DAPI) were from Boster Biotechnology (Wuhan, China).