SiHa and HeLa cells were incubated with primary antibodies overnight at 4 °C. After washing three times, the cells were incubated with R-conjugated Donkey anti-rabbit IgG (1:200 dilution, sc-2095, Santa Cruz Biotechnology, USA) and FITC-conjugated Donkey anti-goat IgG (1:200 dilution, EK033, Zhuangzhibio, China) for 1 h at room temperature. Finally, DAPI Fluoromount-G (SouthernBiotech, USA) was used to counterstain the cell nuclei. The fluorescent was detected, and images were taken by Leica inverted fluorescence microscope. The primary antibodies are listed in Table 2 .
R conjugated donkey anti rabbit igg
Manufactured by Santa Cruz Biotechnology
Sourced in United States
R-conjugated Donkey anti-rabbit IgG is a secondary antibody that binds to rabbit immunoglobulin G (IgG) and is conjugated with a fluorescent R-dye. It is used in immunoassays and other applications that require the detection of rabbit antibodies.
Automatically generated - may contain errors
3 protocols using r conjugated donkey anti rabbit igg
1
Immunofluorescence Staining of SiHa and HeLa Cells
2
Immunofluorescence Staining of SiHa and HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SiHa and HeLa cells were incubated with primary antibodies overnight at 4 °C. After washing three times, the cells were incubated with R-conjugated Donkey anti-rabbit IgG (1:200 dilution, sc-2095, Santa Cruz Biotechnology, USA) and FITC-conjugated Donkey anti-goat IgG (1:200 dilution, EK033, Zhuangzhibio, China) for 1 h at room temperature. Finally, DAPI Fluoromount-G (SouthernBiotech, USA) was used to counterstain the cell nuclei. The uorescent was detected, and images were taken by Leica inverted uorescence microscope. The primary antibodies are listed in table 2.
3
Immunofluorescence Staining of SiHa and HeLa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SiHa and HeLa cells were incubated with primary antibodies overnight at 4 °C. After washing three times, the cells were incubated with R-conjugated Donkey anti-rabbit IgG (1:200 dilution, sc-2095, Santa Cruz Biotechnology, USA) and FITC-conjugated Donkey anti-goat IgG (1:200 dilution, EK033, Zhuangzhibio, China) for 1 h at room temperature. Finally, DAPI Fluoromount-G (SouthernBiotech, USA) was used to counterstain the cell nuclei. The uorescent was detected, and images were taken by Leica inverted uorescence microscope. The primary antibodies are listed in table 2.
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