Naïve CD8 T cells were purified from clone 4 HA-TCR transgenic mice using anti-CD8 beads according to the manufacturer’s instructions (Miltenyi Biotec, Auburn, CA). Cells were then washed twice in cold HBSS and resuspended at 5 × 104 cells/ml in HBSS. Thereafter, 1 × 104 CD8+ T cells were transferred intravenously into B10.D2 mice, which were subsequently infected intraperitoneally with 5 × 106 PFU of VV-HA. 5 days after infection, these infected mice received m-MDSCs or g-MDSCs purified from another cohort of VV-infected B10.D2 mice. Two days later, splenocytes were analyzed for total and IFN-γ+ clonotypic T cells by intracellular cytokine staining.
Anti cd8 beads
Manufactured by Miltenyi Biotec
Sourced in Germany
Anti-CD8 beads are a type of magnetic bead that can be used to isolate CD8+ T cells from cell samples. The beads are coated with antibodies that specifically bind to the CD8 surface protein expressed on CD8+ T cells, allowing for their separation from other cell types using a magnetic field.
Automatically generated - may contain errors
Lab products found in correlation
Facsaria, by BD (2 mentions)
Rapamycin, by Merck Group (1 mentions)
Recombinant ifn γ, by R&D Systems (1 mentions)
Tacrolimus fk506, by Merck Group (1 mentions)
Anti cd4 beads, by Miltenyi Biotec (1 mentions)
Transforming growth factor β tgf β, by R&D Systems (1 mentions)
Ciclosporin a, by Merck Group (1 mentions)
Il 10, by R&D Systems (1 mentions)
Kvprnqdwl, by GenScript (1 mentions)
Macs column, by Miltenyi Biotec (1 mentions)
Anti mouse cd11b beads, by Miltenyi Biotec (1 mentions)
5 protocols using anti cd8 beads
1
Adoptive Transfer of CD8+ T Cells in Vaccinia Virus Infection
2
Regulation of LSP1 Expression in T Cells
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Splenic T cells of WT mice were cultured to examine the major stimuli and their signaling pathways to induce LSP1 expression. Briefly, T cells were isolated from the spleens and prepared as single-cell suspensions. CD4+ T cells or CD8+ T cells were purified by magnetic separation using anti-CD4 beads or anti-CD8 beads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. Purified CD4+ T cells or CD8+ T cells were stimulated with recombinant IFN-γ (10 ng/mL, R&D Systems), transforming growth factor-β (TGF-β, 2 ng/mL, R&D Systems), IL-10 (10 ng/mL, R&D Systems) or antimouse CD3ε Ab (1 µg/mL, 145-2 C11, Invitrogen) plus antimouse CD28 Ab (1 µg/mL, 37.51, Invitrogen) in complete media for 72 hours. In some experiments, ciclosporin A (Sigma), tacrolimus (FK506, Sigma) and rapamycin (Sigma) were treated to the T cells stimulated with anti-CD3/anti-CD28 Abs for 72 hours to determine whether the calcineurin pathway is involved in LSP1 expression. The cultured cells were harvested and stained to detect intracellular LSP1 expression by flow cytometry and/or western blot analysis.
3
Generating Pmel-1 CD8+ T cell-derived iPSCs
4
Adoptive T Cell Transfer and Infection
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Adoptive transfers and infections were performed as described (Smith et al. 2014 (link)). Briefly, CD8+ T cells were isolated from congenically marked adult and neonatal mice, mixed at a 1:1 ratio, and 2 × 104 cells were transferred intravenously into Ly5.2 recipients. The next day, recipients were intravenously infected with Lm-gB (5 × 103 CFU). Effector CD8+ T cells from recipient spleens were isolated with anti-CD8 beads (Miltenyi Biotec) and then sorted via congenic marker expression on a FACS Aria (BD Biosciences).
5
Isolation and Purification of MDSCs and CD8+ T Cells
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MDSCs were isolated from erythrocyte‐depleted splenocytes using antimouse CD11b beads (Miltenyi Biotech, Bergisch Gladbach, Germany) following the manufacturer's instructions. In some experiments, MDSCs were sorted by FACSAria (BD Biosciences). The purity of MDSCs (CD11b+Gr‐1+) was typically >90%. Splenic CD8+ T cells were enriched using anti‐CD8 beads (Miltenyi Biotech). The purity of CD8+ cells was typically >95%.
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