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Baicalein

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Baicalein is a natural compound isolated from the roots of the Scutellaria baicalensis plant. It is a bioactive flavonoid with antioxidant and anti-inflammatory properties. Baicalein is commonly used in biochemical research and biomedical applications, where it serves as a tool for investigating cellular processes and signaling pathways.

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Lab products found in correlation

Apocynin, by Bio-Techne (1 mentions) Tempol, by Bio-Techne (1 mentions) Mito stress test kit, by Agilent Technologies (1 mentions) Seahorse xf dmem medium, by Agilent Technologies (1 mentions) Seahorse xfp extracellular flux analyzer, by Agilent Technologies (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Synthetic rvd1, by Cayman Chemical (1 mentions) Spectramax id5, by Molecular Devices (1 mentions) Celltiter 96 aqueous cell proliferation assay kit, by Promega (1 mentions) Z vad fmk, by Apexbio (1 mentions) Liproxstatin 1, by Bio-Techne (1 mentions) Ferrostatin 1, by Merck Group (1 mentions) Deferoxamine mesylate, by Merck Group (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Triacsin c, by Cayman Chemical (1 mentions)

6 protocols using baicalein

1

Baicalein Treatment in Nos2 Knockout Mice

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Baicalein (Cat. no.1761), from TOCRIS, was given to Nos2-/- mouse at 25mg/kg through Alzet osmotic pumps from 2 days to 28 days' post infection.
Mishra B.B., Lovewell R.R., Olive A.J., Zhang G., Wang W., Eugenin E., Smith C.M., Yao J.P., Long J.E., Dubuke M.L., Palace S.G., Goguen J.D., Baker R.E., Nambi S., Mishra R., Booty M.G., Baer C.E., Shaffer S.A., Dartois V., McCormick B., Chen X, & Sassetti C.M. (2017). Nitric oxide prevents a pathogen permissive granulocytic inflammation during tuberculosis. Nature microbiology, 2, 17072.
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2

Baicalein Treatment in Nos2 Knockout Mice

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Baicalein (Cat. no.1761), from TOCRIS, was given to Nos2-/- mouse at 25mg/kg through Alzet osmotic pumps from 2 days to 28 days' post infection.
Mishra B.B., Lovewell R.R., Olive A.J., Zhang G., Wang W., Eugenin E., Smith C.M., Yao J.P., Long J.E., Dubuke M.L., Palace S.G., Goguen J.D., Baker R.E., Nambi S., Mishra R., Booty M.G., Baer C.E., Shaffer S.A., Dartois V., McCormick B., Chen X, & Sassetti C.M. (2017). Nitric oxide prevents a pathogen permissive granulocytic inflammation during tuberculosis. Nature microbiology, 2, 17072.
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3

Amygdalar Modulation of Oxidative Stress and Behavior

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The rat was anesthetized with pentobarbital sodium (50 mg/kg, i.p.) and placed in a stereotaxic apparatus. A craniotomy over the right amygdala was performed and a guide cannula (outer diameter: 0.64 mm; inner diameter: 0.45 mm) was implanted on the dorsal margin of the CeA, using the following coordinates (in mm): 2.5 caudal to bregma; 4.0 lateral to midline; depth, 7.5. The cannula was fixed to the skull with dental acrylic. The behavioral tests were carried out 5 days after the surgery. For drug application, a microinjection tubing probe was inserted through the guide cannula so that the probe protruded by 1 mm. The probe was connected to a PE-10 polyethylene tube filled with dissolved drug solution. The drug solution was pushed into the brain tissue by injecting 1μl air into the polyethylene tube with a microsyringe. Then the polyethylene tube was heat sealed and the probe was left in place for 10 min before the behavioral tests. The following drugs were used: Tempol, baicalein, apocynin (Tocris). Drugs were dissolved in DMSO (30% in deionized water) and diluted in ACSF (containing [in mM] 117 NaCl, 4.7 KCl, 1.2 NaH2PO4, 2.5 CaCl2, 1.2 MgCl2, 25 NaHCO3 and 11 glucose) at 1:100 to their final concentration for injection into the amygdala.
Lu Y.F., Neugebauer V., Chen J, & Li Z. (2016). Distinct contributions of reactive oxygen species in amygdala to bee venom-induced spontaneous pain-related behaviors. Neuroscience letters, 619, 68-72.
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4

Mitochondrial Function in Murine Endothelial Cells

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Ex vivo mitochondrial function of murine endothelial cells was investigated using an XFp Seahorse extracellular flux analyzer (Agilent, Santa Clara, USA). 30,000 cells were seeded in Seahorse Miniplates in Seahorse XF DMEM medium (103575–100, Agilent, Santa Clara, USA) supplemented with 1% FCS, 2mM L-glutamine, 1mM pyruvate and 5.5mM D-glucose, adjusted to pH 7.4 using 1M NaOH directly after isolation. In some experiments, cells were exposed to 1μM baicalein (Tocris, Bristol, UK), 100nM BCTC (Tocris, Bristol, UK) or DMSO as vehicle control for 2h prior analysis. In other experiments, endothelial cells of in vivo treated mice were used. Miniplates were centrifuged for 20min at 2000g at room temperature. All experiments were performed using the Mito Stress Test Kit (Agilent, Santa Clara, USA, 103010–100) containing oligomycin, FCCP and antimycin/rotenone.
Otto M., Brabenec L., Müller M., Kintrup S., Hellenthal K.E., Holtmeier R., Steinbuch S.C., Karsten O.S., Pryvalov H., Rossaint J., Gross E.R, & Wagner N.M. (2021). Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function. Life sciences, 284, 119925.
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5

Macrophage Infection with Leishmania

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Column purified CD14+ monocytes were obtained from buffy coats from healthy blood donors (from the Hematology and Hemotherapy Foundation of Bahia, HEMOBA), and plated at 2 × 106 cell/well in 24-well plates containing RPMI with 10% fetal bovine serum and treated for 7 d with 50 ng/mL MCSF (Peprotech, Rock Hill, NJ) to differentiate in human macrophages as previously described33 (link). Macrophages were infected (multiplicity of infection 6:1) with stationary phase L. amazonensis promastigotes (MHOM/BR/87/BA336) isolated from a patient with DCL. After 4 h incubation at 37 °C, free parasites were removed by extensive washing with phosphate-buffered saline. Then, indicated concentrations of synthetic RvD1 (Cayman Chemical), with or without Baicalein (a 15-LO inhibitor, TOCRIS, Bristol, UK) were added to cultures. Culture supernatants were collected after 24 h post infection for measurement inflammatory mediators as described above. The intracellular parasite loads were quantified by microscopy and viable promastigotes in Schneider medium. The infectivity index (percentage of infected macrophages x average number of amastigote per macrophage) was determined by randomly counting at least 200 macrophages per slide in light microscope, using the immersion objective (100x).
Malta-Santos H., Andrade B.B., Zanette D.L., Costa J.M., Bozza P.T., Bandeira-Melo C., Barral A., França-Costa J, & Borges V.M. (2017). Resolvin D1 drives establishment of Leishmania amazonensis infection. Scientific Reports, 7, 46363.
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6

UV-Induced Cell Viability Assays

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HEK and melanocytes were plated in 96-wells (1 × 10 [4 (link)] cells/well) 12–16 h at 37C with ferrostatin-1 (Sigma, 10 μM), liproxstatin-1 (Tocris, 1 μM), baicalein (Tocris, 5 μM), triacsin C (Cayman Chem, 10 μM), z-VAD-fmk (Apexbio, 20 μM), deferoxamine mesylate (Sigma, 5 and 10 μM). Plates were exposed to UVA (20 kJ/m2) or UVB (2–10 kJ/m2), incubated 6 h at 37C, and viability was assessed with CellTiter 96 Aqueous Cell Proliferation Assay kit (MTS, Promega) according to manufacturer protocol, by 490 nm absorbance measured with SpectraMax iD5 (Molecular Devices) microplate reader. Alternatively, Fixable Viability Dye eFluor 780 (Invitrogen) was used to assess viability by flow cytometry at 6 h, 24 h and 48 h after irradiation.
Vats K., Kruglov O., Mizes A., Samovich S.N., Amoscato A.A., Tyurin V.A., Tyurina Y.Y., Kagan V.E, & Bunimovich Y.L. (2021). Keratinocyte death by ferroptosis initiates skin inflammation after UVB exposure. Redox Biology, 47, 102143.
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