Overnight cultures were grown by adding a colony from a freshly streaked plate of A. baumannii BAA-1605 to a test tube containing 3 mL of tryptic soy broth (TSB) (Fisher Scientific, DF0370-17-3). This was incubated at 37°C overnight with shaking (120 rpm). The culture was diluted to OD600 = 0.5 and 2.5 μL spotted on the center of a sterile 19-mm polycarbonate membrane (Whatman Nuclepore polycarbonate hydrophilic membranes, Cytiva #10417004). Membranes were sterilized by exposure to UV for 10 min each side and placed (shiny side up) on tryptic soy agar (TSA) plates before spotting. Biofilms were grown on the membranes by incubation at 37°C for 24 hours.
Df0370 17 3
Manufactured by Thermo Fisher Scientific
The DF0370-17-3 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a specific core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on the intended use. Therefore, the description for this product is not available.
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Lab products found in correlation
2 protocols using df0370 17 3
1
Biofilm Formation of A. baumannii
2
GBS Strain A909 Culture Protocol
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GBS strain A909—a serotype Ia, sequence type 7 (ST 7) GBS strain widely used in laboratory investigations—and its derivatives were grown stationary in TS media (Fisher Scientific product number DF0370-17-3) at 28 or 37 °C, supplemented with Erm 5 μg/mL and/or Km 1000 μg/mL as needed for selection. E. coli were maintained in Luria broth (LB) media at 28 or 37 °C, supplemented with Erm 200 μg/mL and/or Km 50 μg/mL as needed for selection. pCAM45 was maintained in E. coli strain JM109. Cloning steps to develop pCAM48 were performed in E. coli strain DH10B.
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