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The H-133 is a laboratory instrument designed for cell culture applications. It provides consistent temperature and humidity control to support the growth and maintenance of cells in a controlled environment.

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Lab products found in correlation

Irdye 800, by LI COR (1 mentions) Irdye 680, by LI COR (1 mentions) H 160, by Santa Cruz Biotechnology (1 mentions) Irdye 680, by Rockland Immunochemicals (1 mentions) Eclipse e600 microscope, by Nikon (1 mentions) Nb300 141, by Novus Biologicals (1 mentions) Ab6672, by Abcam (1 mentions) Glial fibrillary acidic protein (gfap), by Novus Biologicals (1 mentions) Nb100 1028, by Novus Biologicals (1 mentions)

2 protocols using h 133

1

Antibody Validation for Serotonin and PLD

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Primary antibodies include: 5-HT2CR detected by mouse monoclonal D-12 (sc-17797, Santa Cruz; 1:100); 5-HT2CR detected by goat polyclonal N-19 (sc-15081, Santa Cruz; 1:250); PLD1 detected by rabbit polyclonal H-160 (sc-25512, Santa Cruz; 1:100); PLD2 detected by rabbit polyclonal H-133 (sc-25513, Santa Cruz; 1:100); monoclonal mouse anti-b-actin (MAB1501, Chemicon International, Temecula, CA, 1:5000); monoclonal mouse anti pan-cadherin (C-19, Abcam, Cambridge, MA, 1:5000); Phospho-PLD1 (Thr147, pPLD1T147) (#3831, Cell Signaling Technology, Danvers, MA, 1:1000); Phospho-PLD1 (Ser561, pPLD1S561) (#3834, Cell Signaling Technology, Danvers, MA, 1:1000); Phospho-PLD2 (Phospho-Tyr169, pPLD2Y169) (A8400, Assay Biotech, Sunnyvale, CA, 1:1000). Secondary antibodies included infrared-labeled goat anti-mouse (IRDye 680; 926–32220, LI-COR Biosciences, Lincoln, NE); goat anti-rabbit (IRDye 800; 827–08365, LI-COR Biosciences); donkey anti-goat (IRDye 800CW; 605-731-125, Rockland Immunochemicals, Inc., Gilbertsville, PA) and sheep anti-mouse (IRDye 680, Rockland Immunochemicals).
, & Krishnan B. (2016). Amygdala-Hippocampal Phospholipase D (PLD) Signaling As Novel Mechanism of Cocaine-Environment Maladaptive Conditioned Responses. International Journal of Neuropsychopharmacology, 19(6), pyv139.
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2

Immunohistochemical Analysis of Spinal Inflammation

Cited 2 times
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Immunohistochemistry was performed as described previously [24 (link),26 (link)]. Spine axial tissue sections (5 μm) were stained with antibodies for IL1-β (H-133, Santa Cruz Biotechnology 1:100), IL-6 (ab6672, Abcam 1:800), Ionized calcium binding adaptor molecule 1 (Iba-1, NB100–1028, Novus Biologicals; 1:200), or glial fibrillary acidic protein (GFAP, NB300–141, Novus Biologicals, 1:5000). Rabbit IgG was used as a negative control following identical procedures but excluding the primary antibody (Supplementary Figure. 1). Images were captured using a Nikon Eclipse E600 microscope. The same color hue settings were used throughout quantification using NIS element BR imaging software. Several square ROIs (region of interest), with a linear length of 150 μm that adequately represent the entire quantifiable cell area, were used and analyzed individually. A previously optimized object count threshold was loaded and maintained throughout the procedure for quantification of intensity. The average signal intensity was obtained by combing data from three mice with three to five sections each.
Jin L., Xiao L., Ding M., Pan A., Balian G., Sung S.S, & Li X.J. (2021). Heterogeneous macrophages contribute to the pathology of disc herniation induced radiculopathy. The spine journal : official journal of the North American Spine Society, 22(4), 677-689.
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