Isolated embryos were immediately kept in Puck's A Salt Solution after harvesting, fixed in 4% paraformaldehyde at 4 °C overnight, and dehydrated in a sucrose gradient prior to embedding in frozen section compound (Leica). Adult palatal and maxillary tissues were fixed in 10% neutral buffered formalin (Sigma Aldrich, St. Louis, MO) for 24 h at 4 °C and decalcified in 20% EDTA (ethylenediminetetraacetic acid) for 10 days at 4 °C. These tissue samples were then dehydrated in a sucrose gradient prior to embedding in frozen section compound (Leica). Samples were frozen on dry ice and were stored at − 20 °C. Cryosections were cut to 8 μm thickness for subsequent histological analysis.
Frozen section compound
Manufactured by Leica
Sourced in Germany, United States
The Frozen section compound is a product designed for use in cryostat sectioning. It provides a stable, consistent medium for embedding and supporting tissue samples during the frozen sectioning process. The compound is formulated to maintain the integrity of the tissue sample and facilitate the production of high-quality frozen sections for microscopic analysis and further examination.
Automatically generated - may contain errors
Lab products found in correlation
Cm1860, by Leica (6 mentions)
Benzocaine solution, by Merck Group (5 mentions)
Silane coated glass slides, by Muto Pure Chemicals (4 mentions)
3 3 diaminobenzidine (dab), by Vector Laboratories (4 mentions)
Cryostat, by Leica (2 mentions)
Mouse anti rabbit igg hrp, by Santa Cruz Biotechnology (2 mentions)
Neutral buffered formalin, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions)
Rabbit anti ng2 antibody, by Merck Group (1 mentions)
Alexa fluor 546 secondary antibody, by Thermo Fisher Scientific (1 mentions)
Rat anti pdgfrβ, by Thermo Fisher Scientific (1 mentions)
Biorevo, by Keyence (1 mentions)
Paraformaldehyde (pfa), by Avantor (1 mentions)
Normal goat serum (ngs), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Leica (1 mentions)
Vectashield, by Vector Laboratories (1 mentions)
Mas coated glass slide, by Matsunami (1 mentions)
Fluorescence mounting medium, by Agilent Technologies (1 mentions)
Fluorescent stereomicroscope, by Olympus (1 mentions)
4 hydroxynonenal antibody, by Abcam (1 mentions)
41 protocols using frozen section compound
1
Tissue Preparation for Histological Analysis
2
Cryo-sectioning and Histochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues were frozen using a frozen section compound (Leica, Jena, Germany) and sectioned using a model CM 1860 cryotome (Leica). Slide sections were fixed with 10 % formaldehyde and deparaffinized and stained with H&E or Oil Red O stain. Microscopic images were taken under 200 × concentrations using a DFC 480 microscope system (Leica).
3
Pericyte Labeling after Laser Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven days after laser injury, eyes were enucleated and fixed in 4% para-formaldehyde for 1 h on ice, incubated in an increasing concentration of PBS/sucrose (5, 10, and 20%), and embedded in frozen section compound (Leica, Exton, PA, USA). After blocking, the sections were incubated with rat anti-PDGFR-β (1:100, Thermo Fisher Scientific) and rabbit anti-NG2 antibody (1:100, Millipore, USA) and subsequently Alexa Fluor 546 secondary antibodies (1:200, Molecular Probes, Waltham, MA, USA). The specimens were mounted in mounting medium, including DAPI (1:500, Vector Laboratories, Burlingame, CA, USA), and examined under a fluorescence microscope (BIOREVO, Keyence, Tokyo, Japan). The number of nucleus surrounded by NG-2 positive signals, corresponding to pericytes, are counted unbiasedly in five consecutive tissue sections, as previously described [17 (link),18 (link)].
4
Preserving ERK1/2 Activity for In Vivo Imaging
5
Brain Tissue Fixation and Sectioning
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A sexually mature male (n = 1) was anaesthetised by immersion into 0.02% benzocaine solution (Sigma, St. Louis, MO, USA) prior to decapitation. The brain was then harvested and fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.3) for 6 h at 4°C, cryoprotected in 20% sucrose in 0.1 M phosphate buffer (pH 7.3), and embedded in frozen section compound (Leica). The brain was sectioned in sagittal plane (15 μm) using a cryostat (Leica CM1860) and mounted on silane-coated glass slides (Muto Pure Chemicals) and stored at −80°C until use for d-ICC.
6
Tilapia Brain Histology Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male tilapia (n = 2) were anaesthetised using a 0.02% benzocaine solution (Sigma, St. Louis, MO, United States). The fish were then decapitated and the brains harvested. Gonads were examined macroscopically to confirm that the fish were sexually matured. The whole brain samples were then fixed in 4% paraformaldehyde in a 0.1 M phosphate buffer (pH 7.3) for 6 h at 4°C followed by cryoprotection in 20% sucrose in a 0.1 M phosphate buffer (pH 7.3). The cryoprotected whole brain samples were then embedded in frozen section compound (Leica, Wetzlar, Germany) and sectioned coronally at 15 μm using a cryostat (Leica CM1860). The sections were mounted onto silane-coated glass slides (Muto Pure Chemicals, Tokyo, Japan) and stored at -80°C until use for immunocytochemistry.
7
Brain Tissue Preparation for Immunocytochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male tilapia (n = 6) were anaesthetised using a 0.02% benzocaine solution (Sigma, St. Louis, MO, United States). Fish were then decapitated and the brains harvested. Gonads were examined macroscopically to confirm that the fish were sexually matured. The whole brain samples were then fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.3) for 6 h at 4°C followed by cryoprotection in 20% sucrose in 0.1 M phosphate buffer (pH 7.3). The cryoprotected brain samples were then embedded in frozen section compound (Leica, Wetzlar, Germany) and sectioned coronally at 15 μm using a cryostat (Leica CM1860). The coronal sections were divided into two sets and sections that were 30 μm apart were used for double-labelling immunocytochemistry. The sections were mounted onto silane-coated glass slides (Muto Pure Chemicals, Tokyo, Japan) and stored at -80°C until use.
8
Cryosectioning and Fluorescence Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The collected embryos were fixed in 4% PFA in PBS on ice for 30 min. After trimming the embryos, the tissues were dehydrated in 10% sucrose in PBS at 4 °C for 1 h. They were then dehydrated stepwise with 20% sucrose in PBS and 30% sucrose in PBS at 4 °C for 1 h. Tissues were embedded using Frozen Section Compound (Leica, 3801481) and stored at −80°C. Tissues were sectioned at 20 μm thickness using a cryostat (Leica, CM3050). The sections were placed on a MAS-coated glass slide (MATSUNAMI, Japan; S9115) and dried using a slide warmer at 40 °C for 1 h. The glass slides were washed three times with PBS for 1 min. After treatment with 0.1% TritonX-100 in PBS and 1 μg/mL DAPI at room temperature for 10 min, the glass slide was washed with PBS three times for 1 min. The slides were mounted in Fluorescence Mounting Medium (Dako, Agilent, CA, USA; S3023). Fluorescence was observed under the Leica DM2500 fluorescence microscope.
9
Subcutaneous Tumor Implantation and Lymphatic Metastasis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male TβRIIfl/fl and TβRII iΔLEC mice (7–12 weeks old) were subcutaneously implanted with LLCs (2.5 × 105 cells) in 100 μl of PBS. Tumor volumes (V) were calculated using the following formula: length × width × width × 0.5 [13 (link)]. The tumors were surgically removed and embedded into a frozen section compound (Leica). Lymphatic metastasis was measured by the expression of eGFP when LLC-GFP cells implanted into mouse footpad were metastasized. In brief, the legs were clarified with CUBIC 1 week after the LLC-GFP transplantation [14 (link)] and tumor metastasis to the popliteal lymph nodes (PLN) was evaluated with the fluorescent stereomicroscope (Olympus).
10
Oxidative Stress Evaluation in ApoE KO Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ApoE KO and Nox4ApoE DKO mice were received intravenous injection of saline or rFliC (25 μg/mouse). After 2 hrs, the thoracic aorta were isolated and were embedded in Frozen Section Compound (Leica), frozen on dry ice. Five cryosections (each 10 μm thick) were obtained in each animal. The samples were incubated with 4-hydroxynonenal antibody (Abcam) at 4 °C for overnight. An Alexa Fluor® 594-conjugated goat anti-mouse IgG was used as the second antibody.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!