R1 wireless close up flash

Non-clinician research staff conducted the procedures after subject acclimation to room conditions without draughts for least 15 minutes. Subjects were maintained in a calm state throughout. High-resolution digital color images were taken 30 cm perpendicular to the sites (Nikon D90 camera, Micro Nikkor 60-mm lens, Nikon R1 Wireless Close-Up flash, Nikon Inc., Nikon Corporation, Tokyo, Japan).^{45 (link)} Three dimensional (3D) surface images were collected at 60 cm with an Artec MHT scanner (Artec Group, San Diego, CA, USA). Static IR images of IHs and controls were taken 60 cm perpendicular to the sites at an emissivity of 0.98^{16 (link)} (FLIR T400 camera, range 7.5 – 13 μm; uncooled microbolometer focal plan array detector, 320 × 240 pixels resolution; sensitivity < 0.05°C at 30°C; accuracy ± 2°C (<2%), factory calibration, FLIR Systems, Inc., Wilsonville, OR, USA).

Subjects acclimated to room conditions for at least 15 minutes and were maintained in a calm state. High resolution digital color images were collected at 30 cm perpendicular to the contralateral uninvolved skin site (Nikon D90 12.3 megapixels, Micro Nikkor 60-mm lens, cross polarization, and Nikon R1 Wireless Close-Up flash, Nikon Corporation, Tokyo, Japan) (23 (link)). Images were taken with standardized conditions (lighting, subject position), at a fixed distance, and with polarized light (24 (link)) and standardized for white balance and color (23 (link)). Images were color corrected (Macbeth color card), converted from RGB (to CIELab color space, and separated into L* (dark-light), a* (green-red) and b* (blue-yellow) channel images (ImageJ, NIH, Washington, DC, USA) (23 (link)). Mean lightness, red color and yellow color values were reported on 0 – 255 intensity scales.