Fv3000 spectral confocal microscope

Manufactured by Olympus

Sourced in Japan

The FV3000 spectral confocal microscope is a high-performance imaging system designed for advanced biological and materials research. It provides precise and versatile imaging capabilities, allowing users to capture detailed, high-resolution images of a wide range of samples. The FV3000 utilizes spectral detection technology to enable simultaneous acquisition of multiple fluorescence signals, facilitating comprehensive sample analysis.

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Lab products found in correlation

Fluoromount g, by Southern Biotech (4 mentions) Jzl1707 nad p h sensor, by AAT Bioquest (1 mentions) Mntbap, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

FV3000 (1252 citations) Confocal microscope (790 citations) FV3000 confocal microscope (460 citations) FV3000 microscope (111 citations) Confocal FV3000 (4 citations)

4 protocols using fv3000 spectral confocal microscope

Quantifying Nitric Oxide in Tissue

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The NO-specific fluorescent dye 4,5-diaminofluorescein diacetate (DAF-2 DA) was used as a measure of NO. Five µm sections were made from the frozen OCT blocks and stained with 5 μM DAF-2 DA and DAPI in PBS for 30 min in dark. In matched control experiments, the sections were incubated with 100 μM of the NO scavenger, PTIO (Sigma, Burlington, MA, United States) for 10 min prior to DAF-2 DA to confirm the specificity of the fluorescence and prove that signals are derived from NO. The slides were rinsed extensively with PBS, mounted in antifade mounting media Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL, United States), cover slipped, then different fields were captured at ×40 using a confocal microscope (Olympus FV3000 spectral confocal microscope, Tokyo, Japan) with excitation wavelength at 500–530 nm and emission at 590–620 nm, and the fluorescence intensity was analyzed using the microscope provided software.

Hannawi Y., Ewees M.G., Moore J.T, & Zweier J.L. (2022). Characterizing CD38 Expression and Enzymatic Activity in the Brain of Spontaneously Hypertensive Stroke-Prone Rats. Frontiers in Pharmacology, 13, 881708.

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NAD(P)H Fluorescence Imaging Protocol

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JZL1707 NAD(P)H sensor (AAT Bioquest, Sunnyvale, CA, United States) was used to incubate 5 µM section slides at 37°C for 30 min according to manufacturer instructions. The slides were rinsed with PBS, mounted in antifade mounting media Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL, United States), cover slipped, then different fields were captured at 40x using a confocal microscope (Olympus FV3000 spectral confocal microscope, Tokyo, Japan) with excitation wavelength at 540 nm and emission at 590 nm, and the fluorescence intensity was analyzed using the microscope provided software.

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Quantifying Oxidative Stress in VSMCs

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VSMCs were cultured on sterile cover slips in a 6-well plate at a density of 2 × 10⁵ cells/well and incubated in a humidified atmosphere at 37 °C and 5% CO₂ for 4, 24 or 48 hours. Cells were unexposed or exposed to 1.0% TSE, washed with PBS and incubated with the probe dihydroethidium (DHE; 10 μM) and the nuclear stain 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1 μM) in the dark, for 30 min at 37 °C. DHE-derived fluorescence was used to determine O₂^•− generation. Preincubation with or without the SODm (MnTBAP, 100 μM) for 15 minutes before DHE, was performed to confirm that the observed fluorescence was derived from O₂^•− [31 ]. Stained cells on cover slips were rinsed extensively with PBS, mounted in antifade mounting medium Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL), and coverslipped. Different fields were captured at 40x using an Olympus FV3000 spectral confocal microscope (Tokyo, Japan). The fluorescence intensity of the images was analyzed using the software provided by the manufacturer.

Mahgoup E.M., Khaleel S.A., El-Mahdy M.A., Abd-Allah A.R, & Zweier J.L. (2021). Role of Cytoglobin in Cigarette Smoke Constituent-Induced Loss of Nitric Oxide Bioavailability in Vascular Smooth Muscle Cells. Nitric oxide : biology and chemistry, 119, 9-18.

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Superoxide Detection in Brain Sections

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Staining of 5 µM sections of the frozen brains with dihydroethidium (DHE) and DAPI was performed in the dark to determine superoxide generation in the brain sections. Incubation of additional sections with 100 μM of the SODm, MnTBAP (Santa Cruz Biotechnology Inc., Santa Cruz, CA, United States) for 10 min prior to DHE was applied in matched control experiments to confirm the specificity of the fluorescence and prove that signals are derived from superoxide. The slides were rinsed extensively with PBS, mounted in antifade mounting media Fluoromount-G (Southern Biotechnology Associates, Birmingham, AL, United States), cover slipped, then different fields were captured at ×40 using a confocal microscope (Olympus FV3000 spectral confocal microscope, Tokyo, Japan) with excitation wavelength at 495 nm and emission at 515 nm, and the fluorescence intensity was analyzed using the microscope provided software.

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