The measurement of LDH cellular release was performed using the In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based (#TOX7; Sigma-Aldrich, Inc., St. Louis, MO, USA). HUVECs and HaCaT cells were seeded at 2 × 104 and 4 × 104 cells per well, respectively, on tissue culture-treated 96-well plates (Falcon, Corning, NY, USA) and grown for 3 days. Cells were exposed to odorants in reduced serum (1% FBS) media for 1 or 2 h. Hydrogen peroxide (4 mM) exposure was used as a positive control. Absorbance was measured at 490 nm minus background at 690 nm using a microplate-reader (Synergy™ HT, BioTek, Winooski, VT, USA).
Tissue culture treated 96 well plates
Manufactured by BD
Sourced in United States
Tissue culture-treated 96-well plates are a laboratory equipment designed for cell culture applications. These plates provide a standardized, multi-well format for conducting experiments and assays involving cells. The surface of the plates is treated to promote cell attachment and growth, supporting various cell types and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Synergy ht, by Agilent Technologies (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Fix perm cell permeabilization kit, by Thermo Fisher Scientific (1 mentions)
Facsymphony a5, by BD (1 mentions)
Stemspan serum free expansion medium 2, by STEMCELL (1 mentions)
Stem cell factor (scf), by BioLegend (1 mentions)
2 protocols using tissue culture treated 96 well plates
1
Measurement of LDH Release in Cells
2
HSPC Proliferation Assay with OSM
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Briefly, 5,000 HSPCs were sorted directly into tissue culture-treated 96-well plates (Falcon) containing StemSpan Serum-Free Expansion Medium II (SFEM II; STEMCELL Technologies) with penicillin and streptomycin (Pen-Strep, Fisher Scientific) and stem cell factor (SCF, 100 ng/mL, BioLegend), thrombopoietin (TPO, 50 ng/μL, PeproTech), with or without OSM (500 ng/mL, BioLegend) for 24 hours at 37°C and 5% CO 2 . Cells were stained with Ghost UV450 viability dye (Cytek Biosciences) and fixed with the FIX & PERM Cell Permeabilization Kit (Invitrogen) following the manufacturer's protocol. Following fixation, cells were stained with FITC anti-mouse/human Ki-67 (BioLegend) and DAPI. Data were collected on a BD FACSymphony A5.
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