Cryosections (4μm) of spleens from Nr2f6+/+ and Nr2f6−/− immunized mice were fixed with 1% paraformaldehyde for 10 min at room temperature (RT). Slides were then blocked with 2% BSA/PBS for 30 min at RT, followed by incubation with mouse monoclonal antibodies against CD3 (eBioscience 17-0031-82, APC), B220 (eBioscience 12-0425-82, PE) and a biotin-labeled PNA (Vector Labs B-1085) for 45 min at RT. A kit for endogenous biotin and avidin blocking was used according to the manufacturer’s instructions (Vector Labs SP-2002). Subsequently, slides were incubated with streptavidin A488 (Invitrogen S32354) for 45 min at RT. After washing, slides were mounted with Diamond Antifade medium (Invitrogen P36965), images were acquired on a Leica DMi8 inverted microscope, and processed using Fiji software (Schindelin et al., 2012 (link)).
B 1085
Manufactured by Vector Laboratories
The B-1085 is a laboratory equipment product manufactured by Vector Laboratories. It is a core component used in various scientific applications. The product's primary function is to facilitate specific laboratory processes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using b 1085
1
Immunofluorescence Staining of Murine Spleen
2
Immunofluorescent Staining of Murine Spleen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryosections (4μm) of spleens from Nr2f6+/+ and Nr2f6−/− immunized mice were fixed with 1% paraformaldehyde for 10 min at room temperature (RT). Slides were then blocked with 2% BSA/PBS for 30 min at RT, followed by incubation with mouse monoclonal antibodies against CD3 (eBioscience 17-0031-82, APC), B220 (eBioscience 12-0425-82, PE) and a biotin-labeled PNA (Vector Labs B-1085) for 45 min at RT. A kit for endogenous biotin and avidin blocking was used according to the manufacturer’s instructions (Vector Labs SP-2002). Subsequently, slides were incubated with streptavidin A488 (Invitrogen S32354) for 45 min at RT. After washing, slides were mounted with Diamond Antifade medium (Invitrogen P36965), images were acquired on a Leica DMi8 inverted microscope, and processed using Fiji software (Schindelin et al., 2012 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!