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Rabbit anti nucleolin

Manufactured by Abcam
Sourced in United States

Rabbit anti-Nucleolin is a primary antibody that binds to the nucleolar protein nucleolin. Nucleolin is a multifunctional protein involved in various cellular processes, including ribosome biogenesis, chromatin remodeling, and cell proliferation. This antibody can be used to detect and study the expression and localization of nucleolin in different cell types and tissues.

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2 protocols using rabbit anti nucleolin

1

Immunostaining Protocols for Protein Analysis

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Chicken anti-GFP (1:1000, Abcam), Rabbit anti-GFP (1:5000, Invitrogen), mouse anti-hnRNP-A1 (1:1000, Millipore, clone 9H10), Rabbit anti-Nucleolin (1:2000, Abcam, ab22758), Rabbit anti-HDAC4 (1:1000, Abcam, 12172), Mouse anti-TDP43 (1:1000, Abcam, 57105), Mouse anti-γH2AX-pS139 (1:1000, Abcam, 26350), Mouse anti-FLAG (1:2000, Sigma, F3165), Rabbit anti-AAV VP1, VP2, VP3 (1:1000 (WB), 1:50 (ICC), #03-61084, American Res Prod, Waltham, MA), Mouse anti-Integrin αVβ6 (Millipore, MAB2077Z) and mouse anti-NeuN (1:300, Millipore) were used at the dilutions listed above. Alexa Fluor dye (546, 660-C2) maleimide dye (Life Techologies) and Biotin-maleimide (B1267, Sigma) was maintained in aliquots at a stock concentration of 10 mM in DMSO at −20 °C. LC/MS grade acetonitrile (Pierce), ammonium bicarbonate (Sigma), and Pierce™ Trypsin Protease, MS Grade (90057), and formic acid (Sigma) were used for LC/MS described below.
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2

Western Blot Protein Analysis Protocol

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We used a 15% SDS-PAGE gel to isolate proteins, and the proteins were transferred to a PVDF membrane. The primary antibodies used in our study were mouse anti-GAPDH (1:5,000; Santa Cruz, USA), rabbit anti-RPL35 (1:300; ABCAM, UK), rabbit anti-DDX10 (1:300; Proteintech, USA), mouse anti-FLAG (1:2,000; Sigma, USA), rabbit anti-RPL18 (1:1,000; ABCAM, UK), rabbit anti-SRFBP1 (1:2,000; ABCAM, UK), rabbit anti-HNRNPU (1:2,000; Proteintech, USA), rabbit anti-nucleolin (1:1,000; ABCAM, UK), rabbit anti-PRMT5 (1:10,000; ABCAM, UK), goat anti-rabbit IgG (1:2,000; CST, USA), and goat anti-mouse IgG (1:2,000; CST, USA).
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