Total RNA was extracted using Acruturus PicoPure RNA Isolation Kit (Life Technologies). RNA quality was determined with Bioanalyzer 2100 (Agilent). Sequencing libraries were constructed by SMARTer universal low input RNA Kit (Clonetech) according to the manufacturer’s instructions. DNA library samples were submitted to the Stanford Genomics Facility and 100-base paired-end high-throughput sequencing was performed. All sequenced libraries were mapped to the human genome using TopHat and Cufflink56 (link),57 (link) with default parameter setup. Differential expression was analyzed using StrandNGS (AvadisNGS).
Acruturus picopure rna isolation kit
Manufactured by Thermo Fisher Scientific
The Arcturus PicoPure RNA Isolation Kit is a laboratory equipment product designed for the extraction and purification of RNA from small samples. It utilizes a rapid and efficient method to isolate high-quality RNA from limited source material.
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Lab products found in correlation
3 protocols using acruturus picopure rna isolation kit
1
RNA Sequencing Library Preparation
2
RNA-seq Analysis of Human Samples
3
RNA-seq Analysis of Human Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted using Acruturus PicoPure RNA Isolation Kit (Life Technologies). RNA quality was determined with Bioanalyzer 2100 (Agilent). Sequencing libraries were constructed by SMARTer universal low input RNA Kit (Clonetech) according to the manufacturer’s instructions. DNA library samples were submitted to the Stanford Genomics Facility and 100-base paired-end high throughput sequencing was performed. All sequenced libraries were mapped to the human genome using TopHat and Cufflink 42 (link), 43 (link) with default parameter setup. Differential expression was analyzed using StrandNGS (AvadisNGS).
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