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Anti mouse igg or protein a

Manufactured by GE Healthcare

Anti-mouse IgG or protein-A is a laboratory reagent used for the detection and purification of mouse immunoglobulin G (IgG) proteins. It functions by specifically binding to the Fc region of mouse IgG, allowing for the separation and identification of these antibodies in samples.

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2 protocols using anti mouse igg or protein a

1

Antibodies for Western Blotting and Immunofluorescence

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Example 3

Mouse monoclonal antibodies were purchased from Invitrogen (CUL1-2H4C9: #32-2400), Sigma (FLAG-M2: #F3165, β-actin: #A-1978), Covance (HA-16612: #MMS-101P) and Santa Cruz (CD147 8D6: #sc-21746): Rabbit polyclonal antibodies were from Millipore (MCT1: #AB-3538P), Bethyl (DDB1: #A300-462A, CUL4A: #A300-739A), Sigma (FLAG, #F7425), Cell Signaling (α/β-tubulin: #2148, IKZF1: #5443, IKZF3: #12720), Proteintech (CUL4B: #12916-1-AP) and Santa Cruz (HA Y-11: #sc-805. A polyclonal antibody against CRBN was generated by immunizing rabbits with a mixture of two peptides containing amino acids 1-19 and 424-437 of human CRBN (MAGEDQQDAAHNMGNHLPC (SEQ. ID NO: 1) and CPTIDPTEDEISPDK (SEQ ID NO: 2), respectively). Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A coupled with horseradish peroxidase) were from GE Healthcare. Secondary antibodies (anti-rabbit IgG and anti-mouse IgG) coupled to Alexa Fluor 594 or Alexa Fluor 488 for immunofluorescence and flow cytometry were from Life technologies.

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2

Mouse Monoclonal Antibody Characterization

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Example 3

Mouse monoclonal antibodies were purchased from Invitrogen (CUL1-2H4C9: #32-2400), Sigma (FLAG-M2: #F3165, β-actin: #A-1978), Covance (HA-16B12: #MMS-101P) and Santa Cruz (CD147 8D6: #sc-21746). Rabbit polyclonal antibodies were from Millipore (MCT1: #AB-3538P), Bethyl (DDB1: #A300-462A, CUL4A: #A300-739A), Sigma (FLAG, #F7425), Cell Signaling (α/β-tubulin: #2148, IKZF1: #5443, IKZF3: #12720), Proteintech (CUL4B: #12916-1-AP) and Santa Cruz (HA Y-11: #sc-805. A polyclonal antibody against CRBN was generated by immunizing rabbits with a mixture of two peptides containing amino acids 1-19 and 424-437 of human CRBN (MAGEDQQDAAHNMGNHLPC (SEQ ID NO: 1) and CPTIDPTEDEISPDK (SEQ ID NO: 2), respectively). Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A coupled with horseradish peroxidase) were from GE Healthcare. Secondary antibodies (anti-rabbit IgG and anti-mouse IgG) coupled to Alexa Fluor 594 or Alexa Fluor 488 for immunofluorescence and flow cytometry were from Life technologies.

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