Maldi tof toftm 5800 system

The molecular weight of the Tf-MeLioNs sample was analyzed using a MALDI-TOF/TOFTM 5800 system (AB SCIEX, Framingham, MA, USA), with the following operating parameters: operating mode = mass spectroscopy linear mode (positive); mass range (m/z) = 10–140 kDa; matrix (concentration and solution) = sinapinic acid, 10 mg/mL (0.1% trifluoroacetic acid/30% acetonitrile); bovine serum albumin (BSA) preparation = matrix/sample (29/1); sample preparation = matrix/sample (5/1); data processing = baseline correction, noise filter/smooth, and mass calibration.

MRI experiments were conducted at 3.0 T (GE Healthcare, SIGNA Architect, Chicago, IL, USA) and 9.4 T (BioSpec 94/20 USR; Bruker BioSpin, Ettlingen, Germany). Low resolution and high resolution with fast atom bombardment (FAB) ionization (JMS 700 Jeol; Akishima, Tokyo, Japan) was used for the characterization of Cu-DOTA-click-SF, Gd-azido-DOTA, and Gd-DOTA-click-SF formations. Matrix-assisted laser desorption/ionization coupled to time-of-flight mass spectrometry (MALDI-TOF MS) (MALDI-TOF/TOF^TM 5800 system; AB Sciex) was used for the confirmation of the reaction between elastase and Gd-DOTA-click-SF. A SpectraMax^® 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) was used. Inductively coupled plasma coupled to mass spectrometry (ICP/MS; 7700; Agilent, Santa Clara, CA, USA) was used.

The molecular weight of the Tf-MeLioNs sample was analyzed using a MALDI-TOF/TOFTM 5800 system (AB SCIEX, Framingham, MA, USA), with the following operating parameters: operating mode = mass spectroscopy linear mode (positive); mass range (m/z) = 10–140 kDa; matrix (concentration and solution) = sinapinic acid, 10 mg/mL (0.1% trifluoroacetic acid/30% acetonitrile); bovine serum albumin (BSA) preparation = matrix/sample (29/1); sample preparation = matrix/sample (5/1); data processing = baseline correction, noise filter/smooth, and mass calibration.