Dy720

Manufactured by R&D Systems

Sourced in United Kingdom, United States

The DY720 is a lab equipment product designed for research and development purposes. It serves as a tool for performing various experiments and analyses. The core function of the DY720 is to provide a reliable and precise platform for conducting laboratory-based activities. For a detailed and unbiased description of the product's features and capabilities, please consult the technical specifications provided by the manufacturer.

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Lab products found in correlation

7 protocols using dy720

Cerebral Malaria Retinopathy and Biomarkers

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This study is nested in a published study whose objective was to understand the value of malarial retinopathy in cerebral malaria21 (link). Participants included children admitted to Kilifi County Hospital (formally known as Kilifi District Hospital) between July 2005 and December 2011 with a combination of malaria parasites and coma. This means all the children in this study had severe malaria. Retinopathy status was assessed at admission as described previously21 (link). In this study we included all those children who were positive for malaria and had a parasite sample frozen in TRIzol available for var expression analysis. Plasma PfHRP2 concentration was determined as described in Kariuki et al.21 (link). Angiopoietin-2 and soluble ICAM-1 (sICAM-1) plasma levels were determined using commercial ELISA kits DANG20 and DY720 respectively from R&D following manufacturer’s protocol.

Abdi A.I., Kariuki S.M., Muthui M.K., Kivisi C.A., Fegan G., Gitau E., Newton C.R, & Bull P.C. (2015). Differential Plasmodium falciparum surface antigen expression among children with Malarial Retinopathy. Scientific Reports, 5, 18034.

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Quantifying Secreted Osteocalcin and Inflammatory Markers

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Human osteocalcin DuoSet ELISA (R&D Systems; DY1419) was used to measure total secreted osteocalcin in cell culture media. Endothelin Pan Specific, ICAM‐1/CD54, VCAM‐1/CD106, and total MMP‐3 DuoSet ELISAs were performed on cell culture media according to the manufacturer's instructions (Catalog numbers DY1160, DY720, DY809, and DY513; R&D Systems, Abingdon, UK).

Millar S.A., Anderson S.I, & O'sullivan S.E. (2019). Human vascular cell responses to the circulating bone hormone osteocalcin. Journal of Cellular Physiology, 234(11), 21039-21048.

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Quantifying Kidney and Urine Biomarkers

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Mouse TNFα and AREG were measured in kidney lysates using ELISA kits (900-M54 PeproTech and DY989 R&D Systems) according to the manufacturer’s instructions. Human TGFα, HB-EGF, AREG, TNFR1, ICAM1, Axl, and cMET were measured in human urine samples using microsphere-based Luminex Technology and ELISA kits (DY239, DY259, DY262, DY225, DY720, DY154, DY358, respectively; all from R&D Systems). Protein levels were calculated after correction for urine creatinine as described (65 (link)).

Kefaloyianni E., Muthu M.L., Kaeppler J., Sun X., Sabbisetti V., Chalaris A., Rose-John S., Wong E., Sagi I., Waikar S.S., Rennke H., Humphreys B.D., Bonventre J.V, & Herrlich A. (2016). ADAM17 substrate release in proximal tubule drives kidney fibrosis. JCI Insight, 1(13), e87023.

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Biomarker of Endothelial Stress: s-ICAM1 Assay

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Soluble intercellular adhesion molecule-1 (s-ICAM1) was measured as a biomarker of endothelial stress and activation. Elevations in this marker have been documented in several settings of vascular injury (including coronary heart disease), and in relation to SHS exposure.^{13 (link)} Levels of s-ICAM-1 were determined using a sensitive commercially available ELISA kit (R&D systems, Minneapolis, MN; Cat # DY720). Intra and inter- assay variations are less than 5 and 10%, respectively.

Groner J.A., Huang H., Joshi M.S., Eastman N., Nicholson L, & Bauer J.A. (2017). Secondhand smoke exposure and preclinical markers of cardiovascular risk in toddlers. The Journal of pediatrics, 189, 155-161.

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Biomarkers of Thrombosis and Inflammation

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aPL were quantified using Quanta Lite® kits (Inova Diagnostics Inc.). Soluble E-selectin and ICAM-1 were quantified by ELISA (DY724 and DY720, R&D Systems). Myeloperoxidase-DNA complexes were quantified as has been previously described (8 (link)). Cell-free DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P11496). Citrullinated histone H3 was quantified by ELISA (Cayman, 501620). Calprotectin was quantified by ELISA (DY8226, R&D Systems).

Shi H., Zuo Y., Navaz S., Harbaugh A., Hoy C.K., Gandhi A.A., Sule G., Yalavarthi S., Gockman K., Madison J.A., Wang J., Zuo M., Shi Y., Maile M.D., Knight J.S, & Kanthi Y. (2022). Endothelial Cell–Activating Antibodies in COVID‐19. Arthritis & Rheumatology (Hoboken, N.j.), 74(7), 1132-1138.

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Quantifying in vivo NETs and Endothelial Damage

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To quantify in vivo NETs, we measured the circulating nucleosome (histone-DNA) and myeloperoxidase-DNA (MPO-DNA) levels (Cell Death Detection ELISA Plus Kit, Roche Diagnostics) in plasma as in our previous paper. To assess endothelial damage, a commercially available ELISA kit was used for plasma ICAM (Intercellular Adhesion Molecule) -1 (DY720, R&D Systems) and E-Selectin and von Willebrand factor (vWF) (Ray Biotech, USA). We next evaluated their associations and prognostic roles for long-term adverse vascular outcomes.

Lee H.W., Nizet V., An J.N., Lee H.S., Song Y.R., Kim S.G, & Kim J.K. (2021). Uremic serum damages endothelium by provoking excessive neutrophil extracellular trap formation. Scientific Reports, 11, 21439.

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Biomarker Measurement in Plasma Samples

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We determined HRP2 levels from stored plasma of a subset of patients, including all patients admitted in 2009, for a previous study.¹⁴ (link) For patients admitted in 2010 and 2011, TNF, IL10 and sICAM-1 were determined from stored plasma using commercial ELISA kits: (R&D, Minneapolis; DY210, DY217B and DY720).

Mbale E.W., Moxon C.A., Mukaka M., Chagomerana M., Glover S., Chisala N., Omar S., Molyneux M., Seydel K., Craig A.G., Taylor T., Heyderman R.S, & Mallewa M. (2016). HIV coinfection influences the inflammatory response but not the outcome of cerebral malaria in Malawian children. The Journal of Infection, 73(3), 189-199.

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