Biotin conjugated goat anti rabbit polyclonal antibody

Excised tumor and lung tissues were fixed in 4% paraformaldehyde, dehydrated, paraffin-embedded, and cut into sections. Consecutive 4-μm-thick sections were analyzed using primary antibodies against Ki-67 (Cat# ab833, 1:50, Abcam) and a biotin-conjugated goat anti-rabbit polyclonal antibody (1:50; ZSGB-BIO, Beijing, China) as the secondary antibody. Images were obtained by light microscopy (Olympus, Japan) at 100× and 400× magnification and quantified using Image-Pro Plus. Five random fields were examined per animal.

Excised tumor tissues were fixed in 4% paraformaldehyde, dehydrated, paraffin-embedded, and cut into 5 μm sections. The sections were analyzed using primary antibodies against KRAS (1:800, rabbit, Proteintech, China), Ki67 (1:2000, rabbit, Proteintech, China) and a biotin-conjugated goat anti-rabbit polyclonal antibody (1:50; ZSGB-BIO, China) as the secondary antibody. Images were obtained by light microscopy (Olympus, Japan) at 400× magnification.

Excised tumor and adjacent tissues were fixed in 4% paraformaldehyde, dehydrated, paraffin-embedded, and cut into sections. Consecutive 4-μm-thick sections were analyzed using primary antibodies against ZEB1 (human; ab181451; 1:200; Abcam), phospho-STAT3 (human; #9145; 1:100; Cell Signaling Technology), STAT3 (human; #4695; 1:100; Cell Signaling Technology), TWIST-1(human; PA5-49688; 1:100; Invitrogen)and a biotin-conjugated goat anti-rabbit polyclonal antibody (1:50; ZSGB-BIO, Beijing, China) as the secondary antibody. Images were obtained by light microscopy (Olympus, Japan) at 100× and 400× magnification and quantified using Image-Pro Plus. Five random fields were examined per animal.