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Annexin 5 pe

Manufactured by Merck Group
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Sourced in Germany

Annexin V-PE is a fluorescently labeled protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. It is commonly used in flow cytometry and fluorescence microscopy applications to detect and quantify cell death.

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Lab products found in correlation

Facsverse, by BD (2 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Mitochondria extraction kit, by Nanjing Jiancheng (1 mentions)

Spelling variants of this product

Annexin V (119 citations) Annexin V-PE Apoptosis Detection Kit (3 citations) Annexin V-PE and 7-AAD labeling solution (2 citations)

2 protocols using annexin 5 pe

1

Apoptotic Rate in HEK293 Cells

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After treatment with the given drugs for 48 h, the HEK293 cells were stained with 5 µL allophycocyanin (APC; Sigma, Germany) and 5 µL Annexin V-PE (Sigma) for 25 min. The flow cytometry (Becton, Dickinson, and Co., Franklin Lakes, NJ, USA; FACSVerse) was used to detect the cellular apoptotic rate.
Yuan J., Liang X., Zhou W., Feng J., Wang Z., Shen S., Guan X., Zhao L, & Deng F. (2021). TRPA1 promotes cisplatin-induced nephrotoxicity through inflammation mediated by the MAPK/NF-κB signaling pathway. Annals of Translational Medicine, 9(20), 1578.
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2

Mitochondrial Membrane Potential Assay

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The mitochondria of skins were isolated with the Mitochondria Extraction Kit (G006-1-1, Nanjing Jiancheng Bioengineering Institute, China) according to the operating instructions, stained using the mitochondrial membrane potential detection kit (JC-1) (G009-1-3, Nanjing Jiancheng Bioengineering Institute, China) according to operating instructions, and analyzed by flow cytometry (BD FACSVerse) with 5 μl Annexin V-PE (Sigma, Germany) and 5 μl FITC (Sigma, Germany) staining.
Liu X., Yang C., Deng Y., Liu P., Yang H., Du X, & Du Y. (2021). Polygoni Multiflori Radix Preparat Delays Skin Aging by Inducing Mitophagy. BioMed Research International, 2021, 5847153.
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