Cd62p alexa647

The sources of the materials were as follows: calcium-sensitive cell-permeable fluorescent dye Fura-Red-AM, (Molecular Probes, Eugene, OR); LPS O111:B4, LPS O127:B8, ADP, PGI₂, EGTA, HEPES, bovine serum albumin, apyrase grade VII, TRAP-6 (SFLLRN) (Sigma-Aldrich, St Louis, MO); CD62p-Alexa647, CD11b-FITC, CD66b-PE (Sony Biotechnology, San Jose, USA); HBSS, RPMI 1640, Ficoll (PanEco, Moscow, RF), MRS2179, Mes-AMP (Tocris Bioscience, UK); Cysteine-containing version of cross-linked collagen-related peptide (CRP) was kindly provided by Prof. R.W. Farndale (the University of Cambridge, Cambridge, UK). Tyrode’s buffer (150 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, 2 mM CaCl₂, 0.4 mM NaH₂PO₄, 0.4 mM Na₂CO₃, 5 mM HEPES, 5 mM glucose, 0.5% BSA, pH 7.35) was fresh made from reagents (Sigma-Aldrich, St Louis, MO). IBMX was a kind gift of prof. S.P. Gambaryan (SPBU, St. Petersburg, Russia).

Annexin V-Alexa647 and antibodies against P-selectin (CD62p-Alexa647), glycoprotein I (CD42b-PE), integrin αIIβ3 (CD61-PE) and its activation marker (PAC1-FITC) were from Sony Biotechnology (San Jose, CA, USA). Cysteine-containing version of cross-linked collagen-related peptide (CRP) was custom-synthesized and purified by VCPBIO (Shenzhen, China) and then cross-linked. All other reagents were from Sigma-Aldrich (St Louis, MO, USA).