For the detection of Yap1-GFP fluorescence, the cells transformed with pRSII325-Yap1-GFP were grown overnight in SC-Leu, diluted 20 times in fresh SC-Leu and grown for 2 h before cyanidin was added from a sterile stock. Following 2–4 h of cyanidin exposure, live cells were examined with an Olympus fluorescent microscope system (Olympus BX53, Japan) equipped with a HBO-100 mercury lamp and an Olympus DP73 camera. To detect the GFP signals, a GFP filter set (excitation filter 460–480, dichromatic mirror 585, emission filter 495–540) was used. Nuclei were stained with 1 μg/mL 4',6-diamidino-2-phenylindole (DAPI) and observed using DAPI filter set (excitation filter 340–390, dichromatic mirror 410, emission filter 420). The microscopic photographs were processed by CellSens Dimension V1 imaging software (Olympus, Japan). For each strain, one representative image is shown.
Cellsens dimension v1 imaging software
Manufactured by Olympus
Sourced in Japan
CellSens Dimension V1 is an imaging software designed for microscopy applications. It provides core functionalities for image acquisition, processing, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cellsens dimension v1 imaging software
1
Yap1-GFP Fluorescence Detection in Cells
2
Visualizing Copper Uptake in Cells
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To visualize cells grown on agar plates, cells were picked with a sterile loop and gently suspended in 5 μL sterile 10 mM MES–Tris buffer, pH 6.8, directly on the microscope slide. To visualize Cu(I) bound to the cell surface, cells were suspended in 5 μL 10 mM MES–Tris buffer, pH 6.8, containing 1 mM bathocuproine disulfonate (BCS). BCS is a cell-impermeant chelator of Cu(I) with which it forms a blue complex. Bright-field observation of cells was done with an Olympus microscope system (BX53, Olympus, Japan) equipped with an Olympus DP73 camera (Olympus, Tokyo, Japan). Dark-field light scattering by cells or by Cu2O particles was monitored using a dark-field condenser and a 100-W halogen lamp. The microscopic photographs were processed by CellSens Dimension V1 imaging software (Olympus, Tokyo, Japan). For each strain, one representative image is shown.
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