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Sk n be 2

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The SK-N-BE(2) is a cell line derived from a human neuroblastoma. It is a commonly used model for the study of neuroblastoma, a type of cancerous tumor that originates from nerve tissue.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Beyotime (2 mentions) Sh sy5y, by National Collection of Authenticated Cell Cultures (2 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

2 protocols using sk n be 2

1

Culturing Human NB Cell Lines

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Human NB cell lines (SH-SY5Y, and SK-N-BE(2)) were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China), and verified by short tandem repeat analysis. Cells were cultured in DMEM/F12 (Thermo Fisher Scientific) supplemented with 10% FBS (Biological Industries, USA) and 1% penicillin-streptomycin (Beyotime, China) at 37 °C with a humidified atmosphere of 5% CO2 and examined free of mycoplasma regularly.
Zhang Z.M., Cao H.B., Li Z.H., Zhuo R., Tao Y.F., Li X.L., Li G., Liao X.M., Fang F., Xie Y., Wu D., Wang H.R., Wang J.W., Chen Y.L., Yu J.J., Jia S.Q., Yang R.D., Guo X.Y., Yang Y., Feng C.X., Xu Y.Y., Qian G.H, & Pan J. (2022). SAPCD2 promotes neuroblastoma progression by altering the subcellular distribution of E2F7. Cell Death & Disease, 13(2), 174.
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2

Cell Culture of Human Cell Lines

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The human NB cell lines (SK-N-BE(2) and SH-SY5Y) and human 293FT cells were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China). Cells were cultured in DMEM/F12 supplemented with 10% FBS (all from Gibco-BRL, Grand Island, NY, United States) and 1% penicillin‒streptomycin (Beyotime Biotechnology, Shanghai, China) at 37°C in a humidified atmosphere containing 5% CO2.
Yang R., Ma S., Zhuo R., Xu L., Jia S., Yang P., Yao Y., Cao H., Ma L., Pan J, & Wang J. (2022). Suppression of endoplasmic reticulum stress-dependent autophagy enhances cynaropicrin-induced apoptosis via attenuation of the P62/Keap1/Nrf2 pathways in neuroblastoma. Frontiers in Pharmacology, 13, 977622.
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