MicroRNAs were isolated from exosomes using the Total TRIzol Reagent and Protein Isolation Kit (Life Technologies) and were eluted into 200 μL heated elution solution according to the manufacturer’s protocol. The purity and concentration of all RNA samples were quantified spectrophotometrically using the NanoDrop ND‐1000 system (NanoDrop) and the total exosome RNA of all samples over 200 ng.
Total trizol reagent and protein isolation kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
Total TRIzol Reagent and Protein Isolation Kit is a complete solution for the isolation of total RNA, DNA, and proteins from a single sample. The kit combines the guanidinium thiocyanate-phenol-chloroform extraction method with silica-membrane-based purification to provide a simple and efficient way to extract high-quality nucleic acids and proteins from a variety of sample types.
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Lab products found in correlation
2 protocols using total trizol reagent and protein isolation kit
1
Exosomal miRNA Isolation and Quantification
2
Exosomal miRNAs and Protein Extraction
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According to the instructions, the exosomal miRNAs and protein were extracted from serum with the Total TRIzol Reagent and Protein Isolation Kit (Life Technologies, USA). The concentration of the RNA was analyzed using NanoDrop ND-1000 system (NanoDrop, UK). Besides, the concentration of exoaomal protein was detected by BCA kit (Zhuangmeng, Beijing, China).
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