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Phospho iκbα 14d4

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Phospho-IκBα (14D4) is a mouse monoclonal antibody that recognizes IκBα when phosphorylated at serine 32 and serine 36. It is used to detect the phosphorylation of IκBα, which is a key step in the activation of the NF-κB signaling pathway.

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Lab products found in correlation

Ahr d5s6h, by Cell Signaling Technology (1 mentions) Ch 223191, by Selleck Chemicals (1 mentions) Bay 11 7085, by MedChemExpress (1 mentions) Rela d14e12, by Cell Signaling Technology (1 mentions) Iκbα l35a5, by Cell Signaling Technology (1 mentions) α tubulin dm1a, by Cell Signaling Technology (1 mentions) Laminb1 d9v6h, by Cell Signaling Technology (1 mentions) β actin, by Beyotime (1 mentions) Gapdh, by Beyotime (1 mentions) Alexa fluor 647, by Cell Signaling Technology (1 mentions) Phospho p38 3d7, by Cell Signaling Technology (1 mentions) Phospho jnk g9, by Cell Signaling Technology (1 mentions) Gapdh 6c5, by Merck Group (1 mentions)

2 protocols using phospho iκbα 14d4

1

Investigating HTLV-1 Activation Pathway

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CH-223191 was purchased from Selleck. L-kynurenine and BAY11-7085 were purchased from MedChemExpress. Antibodies were used as following: AHR (D5S6H; Cell Signaling Technology), RelA (D14E12; Cell Signaling Technology), IκBα (L35A5; Cell Signaling Technology), phospho-IκBα (14D4; Cell Signaling Technology), α-Tubulin (DM1A; Cell Signaling Technology), LaminB1 (D9V6H; Cell Signaling Technology), HTLV-1 Tax (1A3; Abcam), HTLV-1 gp46 (67/5.5.13.1; Abcam), HTLV-1 p24 (46/3.24.4; Abcam), HTLV-1 p19 (TP-7; Abcam), β-Actin (AF0003; Beyotime Biotechnology), GAPDH (AF0006; Beyotime Biotechnology).
Hong W., Cheng W., Zheng T., Jiang N, & Xu R. (2020). AHR is a tunable knob that controls HTLV-1 latency-reactivation switching. PLoS Pathogens, 16(7), e1008664.
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2

Cellular Protein Analysis by Flow Cytometry and Western Blot

Cited 1 time
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Proteins were detected in permeabilized cells by flow cytometry (gated on live cells) with Alexa Fluor 647, phycoerythrin-, Alexa Fluor 488 or biotin-labeled antibodies to phospho-ERK (E10), phospho-p38 (3D7), phospho-JNK (G9), phospho-IκBα (14D4) (Cell Signaling Technology, Danvers, MA), or antibodies to IRF5 (1H6) (Novus, Littleton, CO), p40phox (B-8), p47phox (A-7), p67phox (D-6) (Santa Cruz Biotechnology, Santa Cruz, CA), NOS2, LC3II (Cell Signaling), phospho-Akt2 or ATG5 (EPR1755) (Abcam, Cambridge, MA). Western blot was performed as per (10 (link)). Blots were incubated with IRF5 (E1N9G), GAPDH (6C5, EMD Millipore) or the antibodies above.
Hedl M., Yan J., Witt H, & Abraham C. (2018). IRF5 is required for Bacterial Clearance in Human M1-Polarized Macrophages and IRF5 Immune-Mediated Disease Risk Variants Modulate This Outcome. Journal of immunology (Baltimore, Md. : 1950), 202(3), 920-930.
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