TP (molecular weight = 360.4 and purity ≥98% by HPLC, as described in the product sheet supplied by Sigma-Aldrich (St Louis, Missouri), catalog No. T3652), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), proteinase inhibitor solution, dimethyl sulfoxide (DMSO), Tiron, and Triton X-100 were purchased from Sigma-Aldrich. Ox-LDL was purchased from the Beijing Solarbio Life Science Company (Beijing, China). RPMI-1640 medium, carboxyfluorescein diacetate succinimidyl ester, fetal bovine serum, and Lipofectamine 2000 were obtained from Invitrogen (Carlsbad, California). Cytokine and chemokine ELISA kits were obtained from R&D Systems (Minneapolis, Minnesota). An 8-isoprostane assay kit was purchased from Cayman Chemical (Ann Arbor, Michigan). Malondialdehyde (MDA) and superoxide dismutase (SOD) activity assay kits were obtained from Beyotime Biotechnology Institute (Shanghai, China). A protein assay kit and RIPA lysis buffer were supplied by Bio-Rad Laboratories (Hercules, California) and Thermo Scientific (Waltham, Massachusetts), respectively.
8 isoprostane assay kit
Manufactured by Cayman Chemical
Sourced in United States
The 8-isoprostane assay kit is a laboratory tool used to quantify the levels of 8-isoprostane, a biomarker related to oxidative stress. The kit provides the necessary reagents and protocols to perform this specific measurement.
Automatically generated - may contain errors
Lab products found in correlation
Gpx assay kit, by Cayman Chemical (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Carboxyfluorescein diacetate succinimidyl ester, by Thermo Fisher Scientific (1 mentions)
Tiron, by Merck Group (1 mentions)
Ripa lysis buffer, by Bio-Rad (1 mentions)
Sod assay kit, by Cayman Chemical (1 mentions)
Victor x4, by PerkinElmer (1 mentions)
3 protocols using 8 isoprostane assay kit
1
Ox-LDL-induced Oxidative Stress Assay
2
Quantifying Oxidative Stress Biomarkers
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Glutathione peroxidase (GPX) activity was measured in the serum by a colorimetric assay kit (GPX Assay kit, Cayman Chemical Company, United States), following the manufacturer’s instructions. 8-Isoprostane levels were measured in the plasma by a colorimetric assay kit (8-Isoprostane Assay kit, Cayman Chemical Company, United States), following the manufacturer’s instructions.
3
Oxidative Stress Biomarkers Assessment
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Superoxide dismutase (SOD) activity and glutathione peroxidase (GPX) activity were measured in the serum by colorimetric assay kits (SOD Assay kit, Cayman Chemical Company, catalog number 706002, USA, and GPX Assay kit, Cayman Chemical Company, catalog number 703102, USA), following the manufacturer's instructions. 8-Isoprostane levels were measured in the plasma by a colorimetric assay kit (8-isoprostane assay kit, Cayman Chemical Company, catalog number 516351, USA), following the manufacturer's instructions. The absorbance was read in a microplate reader (Victor X4, PerkinElmer, Norwalk, Conn., USA) at 450 nm for SOD, 340 nm for GPX, and 412 nm for 8-isoprostane. The range of the SOD assay was from 0.005 to 0.050 U SOD/mL, and intra-assay variation was 3.2%. The intra-assay coefficient of variation of the GPX assay was 5.7%. The sensitivity of the 8-isoprostane assay was 3 pg/mL, and intra-assay variation was 7.2%. All samples were measured in the same assay.
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