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Porcine colipase

Manufactured by Merck Group
Sourced in United Kingdom

Porcine colipase is a laboratory product derived from porcine pancreas. It functions as an enzyme that enhances the activity of pancreatic lipase, a key enzyme involved in the digestion of dietary fats.

Automatically generated - may contain errors

2 protocols using porcine colipase

1

Simulated Digestion of Phospholipids

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Egg L-α-phosphatidylcholine (PC, lecithin grade 1, 99% purity) was obtained from Lipid Products (South Nutfield, Surrey, UK). Porcine gastric mucosa pepsin, bovine α-chymotrypsin, pancreatic α-amylase, porcine colipase, porcine pancreatic lipase and bile salts were obtained from Sigma (Poole, Dorset, UK). Lipase for the gastric phase of digestion was a gastric lipase analogue of fungal origin (F-AP15) from Amano Enzyme Inc. (Nagoya, Japan). All flavonoid and other phytochemical standards were obtained from either Sigma-Aldrich (Poole, UK) or Extrasynthese (Genay, France). All solvents were HPLC grade, water was ultra-pure grade, and other chemicals were of AR quality.
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2

In vitro Digestive Enzyme Protocol

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Egg L-α-phosphatidylcholine (PC, lecithin grade 1, 99% purity) was obtained from Lipid Products (South Nutfield, Surrey, UK). Porcine gastric mucosa pepsin, bovine α-chymotrypsin, pancreatic α-amylase, porcine trypsin, porcine colipase, porcine pancreatic lipase and bile salts were obtained from Sigma (Poole, Dorset, UK). Lipase for the gastric phase of digestion was a gastric lipase analogue of fungal origin from Amano Enzyme Inc. (Nagoya, Japan). All other chemicals were of Analar quality.
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