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Cy2 and cy3 conjugated fluorescent secondary antibodies

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Cy2- and Cy3-conjugated fluorescent secondary antibodies are designed for use in immunoassays and other applications requiring fluorescent detection. These antibodies are produced by conjugating Cy2 or Cy3 dyes to the Fc portion of purified secondary antibodies. The conjugation allows for the visualization and detection of target proteins or molecules in samples.

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Lab products found in correlation

Mouse anti e cadherin, by BD (2 mentions) Biotinylated secondary antibodies for immunohistochemical assays, by Vector Laboratories (2 mentions) Rabbit anti lysozyme, by Agilent Technologies (2 mentions) Mouse anti β catenin, by BD (2 mentions) Sc 15334, by Santa Cruz Biotechnology (1 mentions) Rabbit anti cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions) Pk 6100, by Vector Laboratories (1 mentions) Gfp 1020, by Aves Labs (1 mentions) Rabbit anti mucin 2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ki67, by Vector Laboratories (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Chicken anti gfp, by Aves Labs (1 mentions)

2 protocols using cy2 and cy3 conjugated fluorescent secondary antibodies

1

Antibody Characterization for Immunostaining

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The following antibodies were used for immunofluorescent or immunohistochemical analysis: rabbit anti-Ki67 (VP-K451, 1:1000; Vector Laboratories; Burlingame, CA), rabbit anti-lysozyme (A0099, 1:1000; Dako; Glostrup Municipality, Denmark), mouse anti–E-cadherin (610181, 1:500; BD Transduction Laboratories; San Jose, CA), chicken anti-green fluorescent protein (GFP) for the detection of enhanced yellow fluorescent protein (EYFP) (GFP-1020, 1:2000; Aves Labs; Tigard, OR), rabbit anti-cyclin D1 (CRM 307, 1:100; Biocare Medical; Concord, CA), rabbit anti-cleaved caspase-3 (9661, 1:200; Cell Signaling Technology; Danvers, MA), mouse anti–β-catenin (610153, 1:200; BD Biosciences, San Jose, CA), and rabbit anti–mucin 2 (sc-15334, 1:1000; Santa Cruz Biotechnology; Dallas, TX). Cy2- and Cy3-conjugated fluorescent secondary antibodies were purchased from The Jackson Laboratory and used at a 1:200 dilution. Biotinylated secondary antibodies for immunohistochemical assays (Vector Laboratories) were used at a 1:200 dilution along with the ABC detection system (PK-6100; Vector Laboratories).
Kieckhaefer J.E., Lukovac S., Ye D.Z., Lee D., Beetler D.J., Pack M, & Kaestner K.H. (2016). The RNA Polymerase III Subunit Polr3b Is Required for the Maintenance of Small Intestinal Crypts in Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(6), 783-795.
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2

Immunohistochemistry and Immunofluorescence Analysis

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The following antibodies were used for immunofluorescent or immunohistochemical analysis: rabbit anti-Ki67 (1:1000; Vector Laboratories; Burlingame, CA; VP-K451), rabbit anti-lysozyme (1:1000; Dako; Glostrup Municipality, Denmark; A0099), mouse anti-E-cadherin (1:500; BD Transduction Laboratories; San Jose, CA; 610181), chicken anti-GFP for the detection of YFP (1:2000; Aves Labs; Tigard, OR; GFP-1020), rabbit anti-cyclin D1 (1:100; Biocare Medical; Concord, CA; CRM 307), rabbit anti-cleaved caspase-3 (1:200; Cell Signaling Technology; Danvers, MA; 9661), mouse anti-β-Catenin (1:200; BD Biosciences, San Jose, CA; 610153), and rabbit anti-mucin 2 (1:1000; Santa Cruz Biotechnology; Dallas, TX; sc-15334). Cy2- and Cy3-conjugated fluorescent secondary antibodies were purchased from The Jackson Laboratory and used at 1:200 dilution. Biotinylated secondary antibodies for immunohistochemical assays (Vector Laboratories) were used at a 1:200 dilution along with the ABC detection system (Vector Laboratories; PK-6100).
Kieckhaefer J.E., Lukovac S., Ye D.Z., Lee D., Beetler D.J., Pack M, & Kaestner K.H. (2016). The RNA Polymerase III Subunit Polr3b Is Required for the Maintenance of Small Intestinal Crypts in Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(6), 783-795.
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