Uas br rnai

Manufactured by BD

Sourced in United States

The UAS-br-RNAi is a laboratory equipment product designed for RNA interference (RNAi) experiments. It is a tool used to study gene function and regulation through the targeted silencing of specific genes. The core function of this product is to facilitate the delivery and expression of RNAi constructs in experimental systems.

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Lab products found in correlation

Nre gfp, by BD (2 mentions) Uas arm s10, by BD (1 mentions) Wg lacz, by BD (1 mentions) Uas cd8 rfp, by BD (1 mentions) Uas sa rnai, by BD (1 mentions) En gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas rfp, by BD (1 mentions) En gal4, by BD (1 mentions) Nre gfp, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using uas br rnai

Genetic Manipulation of Drosophila Development

Cited 3 times

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Fly culcture and crosses were performed according to standard procedures. Drosophila stocks used in this study include: UAS-Cut (#36496; Bloomington stock center (BDSC)); FRT19A, cut^C145 (#36496; BDSC); UAS-Wg-HA (#5918; BDSC); UAS-Arm-S10 (#4782; BDSC); UAS-cut-RNAi (#33967; BDSC); UAS-br-RNAi (#27272; BDSC); esg-Gal4, tubP-Gal80^ts, UAS-GFP (Biteau et al., 2008 (link); Micchelli and Perrimon, 2006 (link)); wg-Gal4 (Alexandre et al., 2014 (link)); wg(KO; NRT–Wg) (Alexandre et al., 2014 (link)); UAS-TCF-ΔN (#4785; BDSC); wg-lacZ (#1672; BDSC); Rab3-GFP (#62541; BDSC); UAS-CD8-RFP (#27392, BDSC); cut-intron2-Gal4 (#49818, BDSC); NRE-GFP (#30727; BDSC); UAS-Sgg.S9A (#5255; BDSC); UAS-EcR-DN (#6872; BDSC); UAS-wg-RNAi (#33902; BDSC); UAS-Br-Z1 (#51190; BDSC); UAS-Br-Z3 (#51192; BDSC); UAS-Br-Z4 (#51193; BDSC); FRT19A, dsh³ (#6331; BDSC); UAS-TCF (#4838; BDSC); UAS-Nipped-B-RNAi (#32406; BDSC); UAS-SA-RNAi (#33395; BDSC); UAS-Eip74EF-RNAi (#29353; BDSC); UAS-Eip75B (#26717; BDSC); UAS-white-RNAi (#33623; BDSC); Act5C-FRT>stop>FRT-lacZ.nls (#6355; BDSC).
wg-Gal4 transgenic flies were generated by integrating the Gal4 containing plasmid RIVGal4 (Baena-Lopez et al., 2013 (link)) into the attP site of wg flies (Alexandre et al., 2014 (link)).

Xu K., Liu X., Wang Y., Wong C, & Song Y. (2018). Temporospatial induction of homeodomain gene cut dictates natural lineage reprogramming. eLife, 7, e33934.

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Drosophila Genetic Manipulation Protocol

Cited 1 time

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Strains contain FRT 19A br^npr-3 and hsFLP, FRT 19A RFP (Jia et al., 2014 (link)). The following transgenic lines were used in our study: en-Gal4, UAS-mRFP, NRE-GFP (Bloomington Drosophila Stock Center, BDSC#30729, USA), en-Gal4, UAS-mRFP, NRE-GFP; Gal80ts, UAS-br RNAi (BDSC#27272), UAS-mam RNAi (BDSC#28046), UAS-EcR B1 W650A (BDSC#6872), UAS-br-Z1 (Zhou and Riddiford, 2002 (link)), UAS-Delta RNAi (BDSC#34322), UAS-Delta (BDSC#5614), UAS-NICD (Xie et al., 2014 (link)), cut-lacZ (Jack et al., 1991 (link)) and hsFLP; actin<CD2<Gal4,UAS-RFP/TM3,Sb.
For the FLP/FRT clone induction (Golic and Lindquist, 1989 (link); Xu and Rubin, 1993 (link)), hsFLP, 19A RFP was applied, and clones were induced by one session of heat shock for 2 h at 37°C during late second instar larval stage. To generate mosaic wing discs expressing UAS constructs, the flip-out Gal4 (Pignoni and Zipursky, 1997 (link)) stock hsFLP;actin<CD2<Gal4,UAS-RFP/TM3,Sb was applied. Flip-out clones were induced by 30 min heat shock at 37°C two days before dissection. en-Gal4,UAS-mRFP, NRE-GFP; Gal80ts was used to manipulate gene expression in the posterior compartment of wing discs. In this technique, en-Gal4,UAS-mRFP, NRE-GFP; Gal80ts flies were crossed with UAS lines, and the progeny were raised at 18°C until late second instar larval stage, then shifted to 29°C for two days before dissection.

Jia D., Bryant J., Jevitt A., Calvin G, & Deng W.M. (2016). The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs. Journal of genetics and genomics = Yi chuan xue bao, 43(4), 179-186.

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