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Tris hydroxymethyl aminomethane and neutral red dye

Manufactured by Merck Group
Sourced in Brazil

Tris [hydroxymethyl] aminomethane is a chemical compound used as a buffer in various laboratory procedures. It helps maintain a stable pH environment. Neutral red dye is a pH indicator used for staining and visualization in biological applications.

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2 protocols using tris hydroxymethyl aminomethane and neutral red dye

1

Evaluation of Bioactive Compounds from Diverse Sources

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Benitaka grape (Vitis vinifera L. fruits) was purchased in single batch in a local supermarket. Dimorphandra mollis Beth fava beans were harvested in Private Biological Reserve (22°18′S/47°11′W), in Mogi Guaçu (São Paulo, Brazil), in May 2015. Ruta graveolens leaves were harvested in the Chemical, Biological and Agricultural Pluridisciplinary Research Center—CPQBA (22°48′S/47°0′W) at the University of Campinas (UNICAMP) (Campinas, São Paulo, Brazil) in April 2015. Ginkgo biloba L. dry extract was purchased at Galena (Campinas, São Paulo, Brazil). Ethyl alcohol, acetic acid, hydrochloric acid, boric acid, oxalic acid, acetone, ether, metallic magnesium, metallic zinc, ferric chloride, and aluminum chloride were provided by Synth (São Paulo, Brazil), and 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2-2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH), and fluorescein by Sigma-Aldrich (São Paulo, Brazil). Quercetin (93.3% purity) and rutin (97.3% purity) standard by Acros (Sao Paulo, Brazil). RPMI-1640 and fetal bovine serum were provided by Gibco (Sao Paulo, Brazil) and Tris [hydroxymethyl] aminomethane and neutral red dye was provided by Sigma-Aldrich (São Paulo, Brazil).
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2

Cashew-Based Emulsion Formulation

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Only one cashew batch was purchased in a Brazilian supermarket. The emulsion was formulated using raw materials such as cetearyl alcohol, cetyl alcohol, glycerin, carbomer, dimethicone, polysorbate 80 ® preservative (phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, isobutylparaben) , ethylenediamine tetraacetic acid (EDTA) and butylated hydroxytoluene (BHT) provided by Galena (São Paulo, Brazil) , PharmaSpecial (São Paulo, Brazil) and Lubrizol enterprises (São Paulo, Brazil) . The reagents isopropanol and ethanol were provided by Synth (São Paulo, Brazil), 1,1diphenyl-2-picrylhydrazyl (DPPH), 2-2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), doxorubicin (98.0% of purity) and gentamicin sulfate by Sigma-Aldrich (São Paulo, Brazil), and rutin (97.3% of purity) standard by Acros (Itu, Brazil) . RPMI-1640 and fetal bovine serum were provided by Gibco (Sao Paulo, Brazil) and Tris [hydroxymethyl] aminomethane and neutral red dye was provided by Sigma-Aldrich (São Paulo, Brazil) . Aluminum chloride, hydrochloric acid, acetone, boric acid oxalic acid, ether, ferric chloride, zinc and magnesium metallic by Synth (São Paulo, Brazil) . All chemicals were purchased at analytical grade.
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