Mice from all groups were euthanized, and the cornea and TG were individually harvested. Cornea and TG tissues were digested in complete medium containing 2.5-mg/ml collagenase type IV (Sigma Chemical Co., St. Louis, MO). Digestion was accomplished by incubation at 37°C with shaking for 30 minutes. After digestion, tissues and cells were filtered through a sterile gauze mesh and washed with RPMI 1640 medium. Single-cell suspensions were stained with the following anti-mouse antibodies: CD3 FITC (clone17A2; Biolegend), CD8 PerCP (clone 53–6.7; BD), CD4 PE/Cy7 (clone GK1.5; Biolegend), IFN-γ-PE (clone XMG1.2; BioLegend), CD107a FITC (clone 1D4B; BD), CD107b FITC (clone Ha1/29; BD), and Ki-67 PE/Cy7 (clone 16A8; BioLegend). The surface and the ICS staining protocol were the same as aforementioned. Cells were acquired with the BD FORTESA cytometer.
Cd107a fitc clone 1d4b
Manufactured by BD
CD107a FITC (clone 1D4B) is a laboratory reagent used in flow cytometry applications. It is a fluorescently labeled antibody that binds to the CD107a cell surface antigen, also known as LAMP-1. This reagent can be used to detect and quantify CD107a-expressing cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cd107a fitc clone 1d4b
1
Comprehensive Immune Profiling of Cornea and TG
2
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following anti-mouse antibodies were used: CD3 FITC (clone17A2; Biolegend), CD8 PerCP (clone 53-6.7; BD), CD107a FITC (clone 1D4B; BD), CD107b FITC (clone Ha1/29; BD), IFN-γ-PE (clone XMG1.2; BioLegend), and Ki-67 PE/Cy7 (clone 16A8; BioLegend). Both surface and intracellular staining were performed similarly to the human study as described above.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!