Total RNA was extracted from cell lines via RNA-easy isolation reagent (Vazyme biotech) and was reversed into synthesizing complementary DNA (cDNA) using PrimeScript Mix reagent (Takara). The PCR reaction system was prepared to utilize SYBR Green® Premix Ex Taq™ (Vazyme biotech). Results of individual lncRNAs were normalized to the expression of GAPDH. The primer sequences for each gene are shown in Supplementary Table S2 .
Primescript mix reagent
Manufactured by Takara Bio
PrimeScript Mix is a ready-to-use reagent for reverse transcription and PCR amplification. It contains all the necessary components for cDNA synthesis and subsequent PCR reactions.
Automatically generated - may contain errors
3 protocols using primescript mix reagent
1
RNA Extraction and qPCR Analysis
2
Gene Expression Analysis via qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated from cell samples utilizing RNA-easy isolation reagent (Vazyme Biotech), which was then reversed into cDNA by PrimeScript Mix reagent (Takara). SYBR Green Premix (Vazyme Biotech) was prepared for use in the PCR system. The value of individual genes was finally standardized to the GAPDH expression level. The primer sequences for indicated genes are displayed in Supplementary Table S1 .
3
RNA Extraction and qPCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total cell RNA was extracted by RNA isolation reagent (Takara), then reversed into cDNA by PrimeScript Mix reagent (Takara). SYBR Green Premix (Vazyme biotech) was utilized for PCR reaction system. The value of individual genes was standardized to GAPDH expression level. Supplementary Table S2
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