Human NB cell lines were obtained as follow: GICAN and ACN from Interlab Cell Line Collection, Banca Biologica and Cell Factory (www.iclc.it ), SK-N-AS, SH-SY5Y, SH-EP, SK-N-SH, SK-N-BE(2)c, IMR-32 from the American Type Culture Collection (ATCC), LA-N-1 from Creative Bioarray, LA-N-5 from the Leibniz-Institut DMSZ, SMS-KCNR from Children's Oncology Group Cell Culture, while the Tet-21/N cell line was kindly provided by Dr M. Schwab (University of Heidelberg, Heidelberg, Germany). All NB cell lines were characterized by (i) HLA class I typing by PCR-SSP sets (Genovision) according to the manufacturer's instructions, and (ii) array CGH (see below). The human erythro-leukemia cell line K562 was purchased from ATCC and used as control target for NK cell functional assays. Cells were grown in RPMI 1640 medium supplemented with 10% FBS (Thermo Fisher Scientific), 2 mM glutamine, 100 mg/mL penicillin and 50 mg/mL streptomycin (Euro Clone S.p.a.). Doxycycline (Sigma Aldrich) was used at 10 ng/mL. Lipofectamine 2000 was used, according to manufacturer's instructions (Invitrogen), to transfect SK-N-SH cells with pIRVneoSV empty vector or pIRVneoSV-MYCN, both kindly provided by G. Giannini (“La Sapienza” University of Rome, Italy).
Sk n sh
Manufactured by Thermo Fisher Scientific
Sourced in United States
SK-N-SH is a human-derived neuroblastoma cell line. It is commonly used in research for studying neurobiological processes and neural cell development.
Automatically generated - may contain errors
Lab products found in correlation
Sk n sh, by American Type Culture Collection (11 mentions)
Sh sy5y, by Thermo Fisher Scientific (11 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions)
Sk n be, by Thermo Fisher Scientific (6 mentions)
Penicillin, by Thermo Fisher Scientific (6 mentions)
Streptomycin, by Thermo Fisher Scientific (6 mentions)
Sh sy5y, by American Type Culture Collection (5 mentions)
Imr 32, by American Type Culture Collection (4 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (4 mentions)
Sk n be, by American Type Culture Collection (4 mentions)
Imr 32, by Thermo Fisher Scientific (4 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (4 mentions)
Sk n be 2 c, by American Type Culture Collection (3 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (3 mentions)
Sk n as, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Sk n as, by American Type Culture Collection (2 mentions)
H1299, by Thermo Fisher Scientific (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
24 protocols using sk n sh
1
Characterization of Human Neuroblastoma Cell Lines
2
Characterization of Neuroblastoma Cell Lines
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Tumor samples and matched adjacent noncancerous samples from 30 patients with neuroblastoma were collected, with the patients’ written consent and the agreement of the Children’s Hospital of Fudan University.
Human neuroblastoma SH-SY5Y, SK-N-SH, and NB-1 cell lines were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The SK-N-BE2 cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA, U.S.A.).
SH-SY5Y cells were cultured in MEM-F12 medium (Invitrogen, Carlsbad, CA, U.S.A.) containing 10% fetal bovine serum (FBS) and 1% NEAA (Invitrogen, Carlsbad, CA, U.S.A.). SK-N-SH cells were cultured in MEM medium (Invitrogen, Carlsbad, CA, U.S.A.) containing 10% FBS and 1% NEAA. SK-N-BE2 cells were cultured in MEM-F12 medium containing 10% FBS and 1% NEAA. NB-1 cells were cultured in MEM medium containing 10% FBS and 1% NEAA. All cells were cultured under an atmosphere of 5% CO2 at 37°C.
Human neuroblastoma SH-SY5Y, SK-N-SH, and NB-1 cell lines were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The SK-N-BE2 cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA, U.S.A.).
SH-SY5Y cells were cultured in MEM-F12 medium (Invitrogen, Carlsbad, CA, U.S.A.) containing 10% fetal bovine serum (FBS) and 1% NEAA (Invitrogen, Carlsbad, CA, U.S.A.). SK-N-SH cells were cultured in MEM medium (Invitrogen, Carlsbad, CA, U.S.A.) containing 10% FBS and 1% NEAA. SK-N-BE2 cells were cultured in MEM-F12 medium containing 10% FBS and 1% NEAA. NB-1 cells were cultured in MEM medium containing 10% FBS and 1% NEAA. All cells were cultured under an atmosphere of 5% CO2 at 37°C.
3
Culturing Human Cancer and Normal Cell Lines
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Human cancer cell lines, A2058, AsPC-1, SKNSH, MiaPaCa-2, cfPac-1, NCI-H460, H1915, H1299, U87MG and U373 and the normal pancreatic cell line (HPDE) were obtained from ATCC (Manassas, VA, USA). MDA-MB-231-luc- were obtained from Caliper Life Sciences (Mountain View, CA, USA). HUVEC were from Lonza (Basel, Switzerland). HPDE, A2058, AsPC-1, MiaPaCa-2, cfPac-1, U87MG, Gli36 and U373 were cultured in DMEM (Fisher Scientific, Pittsburgh, PA, USA). H460, H1299 and H1915 were cultured in RPMI 1640 (Fisher Scientific). MDA-MB-231- Luc and SKNSH were cultured in AMEM (Invitrogen, Carlsbad, CA, USA). The above cell lines except HUVEC, were cultured in their respective media supplemented with 10% FBS and 1% Penicillin/Streptomycin. HUVEC were grown in EGM-2 media (Lonza).
4
Cell Culture Protocol for Various Cell Lines
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The mouse liver cell line NCTC 1469, human hepatocyte cell line HL-7702, and human neuroblastoma cell lines BE(2)-M17 and SK-N-SH were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). The chimpanzee skin fibroblast cell line WES was obtained from American Type Cell Culture association (ATCC, Manassas, VA, USA). The human HL-7702, BE(2)-M17, and SK-N-SH, and chimpanzee WES cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% (v/v) fetal bovine serum (Invitrogen), in a humidified 37°C incubator, containing 5% CO 2 . The mouse liver cell line NCTC 1469 was maintained in DMEM supplemented with 10% (v/v) horse serum (Invitrogen) in humidified 37°C and with 5% CO 2 .
5
Culturing Human Neuroblastoma Cell Lines
6
Culturing Human Neuroblastoma Cell Lines
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The human neuroblastoma cell lines SH-SY5Y, IMR-32, SK-N-DZ, SK-N-BE, and SK-N-SH were purchased from ATCC (Manassas, VA). Cells were used within the first 25 passages. SK-N-DZ cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, ATCC), supplemented with non-essential amino acids (Thermo Fisher Scientific, Waltham, MA). SH-SY5Y and SK-N-BE cells were cultured in a 1:1 mixture of Modified Eagle Medium (MEM) and F12 medium, supplemented with non-essential amino acids, and 100 mM sodium pyruvate (all Thermo Fisher Scientific). IMR-32 and SK-N-SH cells were cultured in MEM supplemented with non-essential amino acids, and 100 mM sodium pyruvate (Thermo Fisher Scientific). All media formulations were further supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin, and 100 µg/mL streptomycin (all Thermo Fisher Scientific).
7
Cell Lines and Virus Propagation
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Vero (JCRB9013), Neuro-2a, and SK-N-SH cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (National Institutes of Biomedical Innovation, Health, and Nutrition, Osaka, Japan), and C6/36 cells were obtained from Prof. Koichi Morita at Nagasaki University. The Vero, Neuro-2a, and SK-N-SH cells were maintained in minimum essential medium (MEM, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and the C6/36 cells were maintained in MEM supplemented with 10% FBS and MEM non-essential amino acids (NEAA, Thermo Fisher Scientific, Waltham, MA, USA). The JEV Beijing-1 strain was added to 10% suckling mouse brain homogenates in Hanks’ balanced salt solution containing 20 mM HEPES buffer (pH 8.0) and 02% bovine serum albumin.
8
Cell Line Cultivation and Authentication
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All cell lines, including SK-N-SH, SK-N-BE(2 (link)), SH-SY5Y, SK-N-AS and IMR32, were purchased from Cobioer Biosciences Co., Ltd. The SK-N-SH and IMR32 cells were cultured in minimum essential medium (MEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% 1 mM sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.) and 1% MEM non-essential amino acids (MEM NEAA; Gibco; Thermo Fisher Scientific, Inc.). The SK-N-BE(2 (link)) and SH-SY5Y cells were cultured in MEM/F12 (1:1) (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, 1% 1 mM sodium pyruvate and 1% MEM NEAA. The SK-N-AS cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. All culture mediums were supplemented with 1% penicillin-streptomycin solution (Gibco; Thermo Fisher Scientific, Inc.). All cells were cultured in a 5% CO2 and humidified incubator maintained at 37°C. All cell lines had been authenticated by STR profiling.
9
Establishment of PD Cell Model
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The human neuroblastoma cell line SK-N-SH was purchased from the Shanghai Institute of Life Sciences. The SK-N-SH cells were cultured in high-sugar Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, USA) containing working concentrations of fetal bovine serum and penicillin-streptomycin of 10% and 1%, respectively. Cell culture was carried out in an incubator with the temperature set at 37 °C and a CO2 concentration of 5%. To establish a PD cell model, cells were treated with 0.25, 0.5, or 1 mM 1-methyl-4-phenylpyridinium (MPP+) for 24 hours.
10
Cell Culture and Transfection Protocols
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Human embryonic kidney (HEK293) (KCLB: 21,573), SH-SY5Y (KCLB: 22,266), and SK-N-SH (KCLB: 30,011) cells were purchased from the Korean Cell Line Bank (Seoul, South Korea). SK-N-DZ and SK-N-AS were kindly provided by Prof. Sung-Oh Huh from Hallym University, South Korea. SK-N-DZ, SK-N-AS and HEK293 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (PAN™ BIOTECH, P04-03590, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS) (Gibco BRL, Cat. No. 10,082,147, Rockville, MD, USA) and 1% penicillin and streptomycin (Gibco BRL, Cat. No. 15,140,122, Rockville, MD, USA), while the SH-SY5Y and SK-N-SH cells were maintained in a 1:1 mixture of minimum essential medium (MEM): F12 medium supplemented with 10% FBS and 1% penicillin and streptomycin at 37 °C in a humidified atmosphere with 5% CO2.
The transfection of plasmids and shRNA in the HEK293 cell line was performed using polyethylenimine (Polysciences, Cat. no. 25449-100, Warrington, USA). The SK-N-DZ, SK-N-AS, SH-SY5Y, and SK-N-SH cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific Cat. no. L3000001, USA) according to the manufacturer’s protocol.
The transfection of plasmids and shRNA in the HEK293 cell line was performed using polyethylenimine (Polysciences, Cat. no. 25449-100, Warrington, USA). The SK-N-DZ, SK-N-AS, SH-SY5Y, and SK-N-SH cells were transfected with Lipofectamine 3000 (Thermo Fisher Scientific Cat. no. L3000001, USA) according to the manufacturer’s protocol.
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