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Ab1778 is a laboratory product manufactured by Santa Cruz Biotechnology. It is a research-use antibody that can be used for various applications in life science research. The core function of Ab1778 is to serve as a tool for detecting and studying specific molecular targets within biological samples.

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Lab products found in correlation

Ab13970, by Abcam (2 mentions) Sc 313, by Proteintech (2 mentions) Sc 1675, by Merck Group (2 mentions) Ab4729, by Abcam (2 mentions) Abn78, by Merck Group (1 mentions) Ab183024, by Abcam (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Prb 278p, by Merck Group (1 mentions) Anti calbindin, by Merck Group (1 mentions) Anti p27, by BD (1 mentions) Ab13970, by Cell Signaling Technology (1 mentions) Anti pax2, by Thermo Fisher Scientific (1 mentions) Sc 17320, by BD (1 mentions) Anti sox2, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Cell Signaling Technology (1 mentions)

4 protocols using ab1778

1

Characterization of Transcription Factors

Cited 2 times
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Antibodies to Calbindin (Millipore ab1778; IHC), Mef2a (Santa Cruz sc-313; ChIP/IP/IB), Mef2c (Protein-Tech 18290-1-AP; IB), 14-3-3 (Santa Cruz sc-1675; IB), Cre (Millipore 69050-3; IB), histone H3K27ac (Abcam ab4729; ChIP), cleaved caspase 3 (Cell Signaling Technology 9661S; IHC), GFP (Abcam ab13970; IHC) were purchased. Antibodies to Mef2a (IB) and Mef2d (ChIP/IP/IB) have been described (Flavell et al., 2008 (link); Andzelm et al., 2015 (link)).
Majidi S.P., Reddy N.C., Moore M.J., Chen H., Yamada T., Andzelm M.M., Cherry T.J., Hu L.S., Greenberg M.E, & Bonni A. (2019). Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors. Cell reports, 29(7), 2001-2015.e5.
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2

Comprehensive Antibody Characterization for CLIP, IF, and WB

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Primary antibodies used for CLIP, immunofluorescent staining (IF), and western blots (WB) were: mouse anti-GFP ((Heiman et al., 2014 (link)), clones 19F7 and 19C8, cTag-CLIP), rat anti-GFP (nacalai tesque, GF090R, IF: 1/1,000), rabbit anti-Cux1 (Santa Cruz, sc-13024, IF: 1/200), mouse anti-GFP (Santa Cruz, sc-9996, WB: 1/1,1000), rabbit anti-mRFP/tdTomato (ROCKLAND, 600-401-379, IF: 1/2,000), rabbit anti-NeuN/RBFOX3 (Millipore, ABN78, IF: 1/1,000), guinea pig anti-NeuN/RBFOX3 (Millipore, ABN90P, IF: 1/1,000), goat anti-NOVA2 (Santa Cruz, sc-10546, IF; 1/500, WB; 1/2,000, CLIP), rabbit anti-NOVA1 [EPR13847] (abcam, ab183024, IF: 1/1,1000), human anti-pan NOVA (anti-NOVA paraneoplastic human serum, WB: 1/5,000), rabbit anti-PTBP2 (Polydorides et al., 2000 (link), IF:1/5,000, CLIP), guinea pig anti-vGlut1 (Synaptic System, 135 304, IF:1/2,000), rabbit anti-vGlut2 (Synaptic System, 135 403, IF: 1/2,000), mouse anti-VGAT (Synaptic System, 131 011, IF:1/1,000), rabbit anti-Calbindin D28 (Millipore, AB1778, IF: 1/1,000), goat anti-Calbindin D28 (Santa Cruz, sc-7691, IF: 1/500), rabbit anti-ITPR1/InsP3R, type1 (Millipore, ABS55, IF:1/2,000), mouse anti-GAPDH (Santa Cruz, sc-32233, WB: 1/1,000).
Saito Y., Yuan Y., Zucker-Scharff I., Fak J.J., Jereb S., Tajima Y., Licatalosi D.D, & Darnell R.B. (2019). Differential NOVA2-mediated splicing in excitatory and inhibitory neurons regulates cortical development and cerebellar function. Neuron, 101(4), 707-720.e5.
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3

Characterization of Transcription Factors

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to Calbindin (Millipore ab1778; IHC), Mef2a (Santa Cruz sc-313; ChIP/IP/IB), Mef2c (Protein-Tech 18290-1-AP; IB), 14-3-3 (Santa Cruz sc-1675; IB), Cre (Millipore 69050-3; IB), histone H3K27ac (Abcam ab4729; ChIP), cleaved caspase 3 (Cell Signaling Technology 9661S; IHC), GFP (Abcam ab13970; IHC) were purchased. Antibodies to Mef2a (IB) and Mef2d (ChIP/IP/IB) have been described (Flavell et al., 2008 (link); Andzelm et al., 2015 (link)).
Majidi S.P., Reddy N.C., Moore M.J., Chen H., Yamada T., Andzelm M.M., Cherry T.J., Hu L.S., Greenberg M.E, & Bonni A. (2019). Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors. Cell reports, 29(7), 2001-2015.e5.
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4

Cerebellum tissue preparation and immunohistochemistry

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Cerebella of mice were formalin-fixed and paraffin-embedded (8 μm sections; see above). Alternatively, brains were fixed with 4% PFA in PBS for 2.5 hours on ice, followed by cryoprotection with 30% sucrose in PBS over night. Fixed tissues were embedded in O.C.T™ Compound (Sakura), frozen on dry ice and cryopreserved at -80°C for cryosections (12μm thickness). For staining with anti-PCNA antibody, sections were subjected to heat antigen retrieval with 10 mM citrate buffer for 30 minutes. After blocking with 10% normal donkey serum in PBS-T (0,1% Triton X-100 in PBS) for 30 minutes at room temperature, sections were incubated with primary antibody over night at 4°C. Incubation with secondary antibody was performed for 30 minutes at room temperature. Subsequently, sections were mounted using the ProLong Gold antifade reagent (Lifetechnologies). The antibodies used for IHC were anti-Pax6 (1:1000, Covance, PRB-278P), anti-PCNA (1:1000, Merck Millipore, NA03), anti-GFP (1:1000, Abcam, ab13970), anti-GFP (1:1000, Cell Signaling, 2555), anti-Pax2 (1:1000, Invitrogen, 71-6000), anti-Calbindin (1:1000, Millipore, AB1778), anti-Sox2 (1:500, Santa Cruz, sc-17320), anti-p27 (1:1000, BD Biosciences, 610242) and anti-Atoh1 (1:1000, Muguruma et al., 201042 (link)).
Zuckermann M., Hovestadt V., Knobbe-Thomsen C.B., Zapatka M., Northcott P.A., Schramm K., Belic J., Jones D.T., Tschida B., Moriarity B., Largaespada D., Roussel M.F., Korshunov A., Reifenberger G., Pfister S.M., Lichter P., Kawauchi D, & Gronych J. (2015). Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nature Communications, 6, 7391.
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