32p atp and t4 polynucleotide kinase

Manufactured by Thermo Fisher Scientific

Sourced in United States

[32P]-ATP and T4 polynucleotide kinase are commonly used laboratory reagents. [32P]-ATP is a radioactive form of adenosine triphosphate (ATP) that is labeled with the radioactive isotope phosphorus-32 (32P). T4 polynucleotide kinase is an enzyme that catalyzes the transfer of the gamma-phosphate group from ATP to the 5' hydroxyl terminus of DNA or RNA molecules.

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T4 polynucleotide kinase (323 citations) Polynucleotide kinase (27 citations) T4-kinase (17 citations)

3 protocols using 32p atp and t4 polynucleotide kinase

5'-end Labeling of miRNAs

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5′-end labelling using [³²P]-ATP and T4 polynucleotide kinase (Thermo Scientific, Waltham, MA, USA) and isolation of [³²P]-miRNAs miR-21, miR-21_1, miR-17, miR-18a, miR-155 (Table S5) were carried out according to procedure previously described in [26 (link),66 (link)].

Patutina O., Chiglintseva D., Bichenkova E., Gaponova S., Mironova N., Vlassov V, & Zenkova M. (2020). Dual miRNases for Triple Incision of miRNA Target: Design Concept and Catalytic Performance. Molecules, 25(10), 2459.

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5'-End Labeling of miRNAs

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The 5′-end labelling using [³²P]–ATP and T4 polynucleotide kinase (Thermo Scientific, Waltham, MA, USA) and the isolation of synthetic single-stranded [³²P]-miRNAs miR-21–5p 5′-UAGCUUAUCAGACUGAUGUUGA-3′ and miR-17–5p 5′-CAAAGUGCUUACAGUGCAGGUAG-3′ were carried out according to a procedure previously described [55 (link),56 (link)].

Patutina O., Chiglintseva D., Amirloo B., Clarke D., Gaponova S., Vlassov V., Bichenkova E, & Zenkova M. (2022). Bulge-Forming miRNases Cleave Oncogenic miRNAs at the Central Loop Region in a Sequence-Specific Manner. International Journal of Molecular Sciences, 23(12), 6562.

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Radiolabeling of miR-21 miRNA

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MiR-21 5'-UAGCUUAUCAGACUGAUGUUGA-3' was synthesized by Dr. Maria I.
Meschaninova (ICBFM SB RAS). 5'-end labelling using [ 32 P]ATP and T4 polynucleotide kinase (Thermo Scientific, USA) and isolation of [ 32 P]-miR-21 was carried out according to procedure previously described in [55, 77] .

Patutina O.A., Bichenkova E.V., Miroshnichenko S.K., Mironova N.L., Trivoluzzi L.T., Burusco K.K., Bryce R.A., Vlassov V.V, & Zenkova M.A. (2017). miRNases: Novel peptide-oligonucleotide bioconjugates that silence miR-21 in lymphosarcoma cells. Biomaterials, 122.

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