Fluorescence activated cell sorting (FACS) was carried out as described previously in our paper [20 (link)]. The following antibodies were used to mark the cell surface epitopes: CD90-phycoerythrin (PE), CD44-PE, CD73-PE, CD166-PE and CD34-PE, CD45-fluoroisothyocyanate (FITC), and HLA-DR-FITC (all from BD Pharmingen). All analyses were standardized against negative control cells incubated with isotype specific IgG1-PE and IgG1-FITC (BD Pharmingen). At least 10,000 events were acquired on Guava Technologies flow cytometer, and the results were analyzed using Cytosoft, Version 5.2 (Guava Technologies).
Cd90 phycoerythrin pe
Manufactured by BD
Sourced in United States
CD90-phycoerythrin (PE) is a fluorescent dye conjugate used for cell surface marker detection in flow cytometry applications. It binds to the CD90 antigen expressed on certain cell types.
Automatically generated - may contain errors
Lab products found in correlation
Igg1 pe, by BD (2 mentions)
Cd73 pe, by BD (2 mentions)
Hla dr fitc, by BD (2 mentions)
Igg1 fitc, by BD (2 mentions)
Cytosoft version 5, by Merck Group (2 mentions)
Cd44 pe, by BD (1 mentions)
Cd34 pe, by BD (1 mentions)
Cd166 pe, by BD (1 mentions)
Cd45 fluoroisothyocyanate fitc, by BD (1 mentions)
Annexin 5 cy5, by BD (1 mentions)
Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions)
Cd45 apc h7, by BD (1 mentions)
Cd166 pe and cd34 pe, by BD (1 mentions)
4 protocols using cd90 phycoerythrin pe
1
Immunophenotyping of Cell Populations
2
Apoptosis Measurement in AML Cells
3
Immunophenotyping of Deciduous DPSCs
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Immunophenotyping of deciduous DPSCs were examined using flow cytometry at passage 5 [22 (link)]. The antibodies used to mark the cell surface epitopes were CD90-phycoerythrin (PE), CD73-PE, CD166-PE and CD34-PE, CD45-fluoroisothiocyanate (FITC), and HLA-DR-FITC (all from BD Pharmingen, San Jose, CA, USA). All analyses were standardized against negative control cells incubated with isotype-specific immunoglobulin (Ig) G1-PE and IgG1-FITC (BD Pharmingen). At least 10,000 events were acquired on a Guava Technologies flow cytometer, and the results were analysed using Cytosoft, Version 5.2 (Guava Technologies, Hayward, CA, USA).
4
MSC Surface Marker Expression Analysis
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MSCs were cultivated at a cell density of 1.0 × 105 cell/mL under standard cultivation conditions as described above. After 48 h of incubation, they were washed with warm PBS and trypsinized, and the cells were pelleted by centrifugation at 1200 rpm for 10 min. The cells were fixed in warm 4% paraformaldehyde (PFA), washed with PBS and stained with fluorescence-labeled antibodies (Abs) with incubation at 4 °C for 30 min. The used antibodies were specific for CD44 directly conjugated with fluorescein isothiocyanate (FITC) (1:100, Mouse, BD Pharmingen, USA), CD73- allophycocyanin (APC) (1:100, mouse, BioLegend, USA) and CD90- phycoerythrin (PE) (1:100, Mouse, BD Pharmingen, USA). Labeled cells were analyzed using a Fluorescence-activated cell sorting (FACS) instrument, and the results were depicted in histogram graphics.
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