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Splint oligonucleotides

Manufactured by Integrated DNA Technologies
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Splint oligonucleotides are synthetic DNA molecules designed to join two or more nucleic acid strands together. They serve as linkers or connectors in various molecular biology applications.

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Lab products found in correlation

Biotinylated extension oligonucleotides, by Integrated DNA Technologies (2 mentions) Hplc purified fluorescent labeled primers, by Integrated DNA Technologies (2 mentions)

Close spelling variants of this product

DNA as splint oligonucleotide Oligonucleotides

2 protocols using splint oligonucleotides

1

Synthesis and Characterization of DNA Interstrand Crosslinks

Cited 1 time
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The sequences of the oligonucleotides were listed in Supplementary Table S1. HPLC-purified fluorescent labeled primers, biotinylated extension oligonucleotides, and splint oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). Single-stranded oligonucleotides with ICL precursors, 39mer 20-bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6)-ICL), and 39mer 6-bp ICL (39+6-ICL) were synthesized and purified as previously reported (37 (link)). The identity of the ICLs was confirmed by LC–MS (Supplementary Figure S1A).
Bezalel-Buch R., Cheun Y.K., Roy U., Schärer O.D, & Burgers P.M. (2020). Bypass of DNA interstrand crosslinks by a Rev1–DNA polymerase ζ complex. Nucleic Acids Research, 48(15), 8461-8473.
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2

Synthesis and Characterization of ICL Oligonucleotides

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The sequences of the oligonucleotides were listed in Table S1. HPLC-purified fluorescent labeled primers, biotinylated extension oligonucleotides, and splint oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA). Single-stranded oligonucleotides with ICL precursors, 39mer 8 atom 20 bp ICL that contains two franking uracil bases around the crosslinked base (39+20(6) DMEDA ICL), and 39mer 8 atom 6 bp ICL (39+6 DMEDA ICL) were synthesized and purified as previously reported (33) . The identity of the ICLs was confirmed by
Bezalel‐Buch R., Cheun Y., Roy U., Schärer O.D., & Burgers P. (2020). Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex.
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