U2OS, A549, MCF10A, and MDA-MB-231 cells were obtained from ATCC and maintained at low passage numbers (generally less than 20 passages from the original vial). U2OS, A549, and MDA-MB-231 cells were grown in Dulbecco’s modified eagles medium (DMEM) supplemented with 10% FBS, 2 mM glutamine, and penicillin/streptomycin. MCF10A cells were grown in DMEM/F12 media, supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 μg/ml insulin, and penicillin/streptomycin. Drugs were purchased from either Selleck Chemicals, APEXBio Technology, or Sigma-Aldrich. SYTOX green was purchased from Thermo-Fisher. Poly/Mono ADP Ribose Rabbit mAb (83732) was purchased from Cell Signaling Technologies. Purified rabbit anti-active caspase 3 (559565) and Alexa Fluor® 647 conjugated mouse anti-cleaved PARP (558710) were purchased from BD Pharmingen. Secondary antibodies were purchased from LICOR Biosciences and BD Biosciences. See also Supplementary Table 2 .
Purified rabbit anti active caspase 3 559565
Manufactured by BD
Purified rabbit anti-active caspase 3 (559565) is a laboratory reagent used for the detection of active caspase-3 in various biological samples. It is a purified polyclonal antibody raised in rabbits against the active form of caspase-3.
Automatically generated - may contain errors
2 protocols using purified rabbit anti active caspase 3 559565
1
Cell Culture and Reagent Preparation for Assays
2
Cell Culture and Reagent Preparation for Assays
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U2OS, A549, MCF10A, and MDA-MB-231 cells were obtained from ATCC and maintained at low passage numbers (generally less than 20 passages from the original vial). U2OS, A549, and MDA-MB-231 cells were grown in Dulbecco’s modified eagles medium (DMEM) supplemented with 10% FBS, 2 mM glutamine, and penicillin/streptomycin. MCF10A cells were grown in DMEM/F12 media, supplemented with 5% horse serum, 20 ng/ml EGF, 0.5 mg/ml hydrocortisone, 100 ng/ml cholera toxin, 10 μg/ml insulin, and penicillin/streptomycin. Drugs were purchased from either Selleck Chemicals, APEXBio Technology, or Sigma-Aldrich. SYTOX green was purchased from Thermo-Fisher. Poly/Mono ADP Ribose Rabbit mAb (83732) was purchased from Cell Signaling Technologies. Purified rabbit anti-active caspase 3 (559565) and Alexa Fluor® 647 conjugated mouse anti-cleaved PARP (558710) were purchased from BD Pharmingen. Secondary antibodies were purchased from LICOR Biosciences and BD Biosciences. See also Supplementary Table 2 .
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